PowerWater® Sterivex™ DNA Isolation Kit

Overview

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Features

• Fast
  
  • 40 minute protocol from start to finish

• In-unit digestion
  • No need to struggle with removing filters from Sterivex™ Units

• No harsh chemicals
  • Column-based purification protocol eliminates the need for phenol/chloroform extraction.

• Cell Release Solution
  
  • Improves release of cells from the membrane which increases DNA yield

Water For Life Program - MO BIO will donate 1% of the sale of this product to Water.org.

Description

The PowerWater® Sterivex™ DNA Isolation Kit is the only kit available to isolate genomic DNA from Millipore's Sterivex™ filter units without the need for enzymes or hazardous organic chemicals.  Utilizing a novel Cell Release Solution, microbes are released from the Sterivex™ filter units without extensive incubation times or having to cut open the plastic casing holding the membrane, enabling purification of high quality DNA in just 40 minutes. Patented Inhibitor Removal Technology® (IRT) is included to provide high quality DNA from all types of water samples, even those containing heavy amounts of contaminants.  In addition, a unique silica binding column and column extender enable one-step addition of a large volume of solution (4.5ml) and elution in a 50-100 µl volume. 

The PowerWater® Sterivex™ DNA Isolation Kit protocol starts with the addition and incubation of the Sterivex™ units with Cell Release Solution. Lysis buffer is then added to the units, followed by mixing and removal of the lysate for homogenization in a 5 ml bead beating tube.  After the protein and inhibitor removal steps, total genomic DNA is captured on a silica binding column using a vacuum manifold. High quality DNA is then washed and eluted from the binding column membrane for use in downstream applications including PCR and qPCR. This kit is recommended for use with Millipore catalog # SVGPL10RC Sterivex™ GP Filter units, but is also compatible with other Sterivex™ Filter units.

Sample Kit

Click to try a sample.

Research and Development

Time-Saving Protocol

High Yield and Purity DNA

DNA isolated using the PowerWater® Sterivex™ DNA Isolation kit exhibited a higher yield of high MW, dsDNA with less RNA carryover compared with other common in-unit or out-of-unit lysis protocols followed by phenol/chloroform extraction.

Inhibitor-Free DNA

DNA isolated using the PowerWater® Sterivex™ DNA Isolation kit is free of PCR inhibitors, while DNA purified using an in-unit lysis protocol followed by phenol/chloroform extraction exhibited PCR inhibition and failed to amplify without a 1:10 dilution.
 

MSDS

14600

Protocol

14600

Specifications

Storage Conditions

Kit Components