UltraClean® Tissue & Cells RNA Isolation Kit

Overview

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Features

- Rapid protocol isolates total RNA from animal tissues or cells in 20 minutes

- Yields up to 60 μg high quality RNA for RT and RT-PCR

- Complete kit includes all required RNase-free tubes and ready to use reagents

- No phenol/chloroform extraction, no CsCl gradients and no ethanol precipitation

Description

The UltraClean^® Tissue RNA Isolation Kit is designed to provide efficient purification of total RNA from a variety of mammalian tissues or cultured cells.

Prepared fresh or frozen tissues are homogenized using a tissue homogenizer or mortar and pestle in the presence of lysis solutions. The RNA is then bound to a silica spin filter, washed and eluted into certified RNase-free water (provided). Kit includes all RNase-free reagents ready to use and all required spin filters and tubes. RNA is of high quality and is ready for any downstream applications such as RT or RT-PCR.

Requires our Vortex Adapter for maximum efficiency.

Sample Kit

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Research and Development

Total RNA From Mouse Tissues

MSDS

15000

Protocol

15000

Specifications

Storage Conditions

Publications

• Prabha, S. and Verghese, S. Existence of Proviral Porcine Endogenous Retrovirus in Fresh and Decellularised Porcine Tissues. Indian Journal of Medical Microbiology. 26(3): 228-232 (2008).
• Endara, P., Trueba, G., Solberg, O.D., Bates, S.J., Ponce, K., Cevallos, W., Matthijnssens, J., Eisenberg, J.N. Symptomatic and Subclinical Infection With Rotavirus P[8]G9, Rural Ecuador. Emerging Infectious Diseases. 13(4): 574-80 (2007).
• Blesa, S., Cortijo, J., Mata, M., Serrano, A., Closa, D., Santangelo, F., Estrela, J.M., Suchankova, J., Morcillo. E.J. Oral N-acetylcysteine Attenuates The Rat Pulmonary Inflammatory Response To Antigen. European Respiratory Journal. 21(3): 394-400 (2003).

Kit Components