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PowerLyzer™ UltraClean® Tissue & Cells RNA Isolation Kit
Overview
For isolation of total RNA from mammalian tissues or cultured cells, optimized for use with bead based homogenizers such as the PowerLyzer™ 24.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 15055-50 | $179.00 |
|
Features
- Rapid protocol isolates total RNA from animal tissues or cells in 10 minutes
- Optimized for use with bead based homogenizers such as the PowerLyzer™ 24
- Yields up to 60 μg high quality RNA for RT and RT-PCR
- Complete kit includes all required RNase-free tubes and ready to use reagents
- No phenol/chloroform extraction, no CsCl gradients and no ethanol precipitation
Description
The PowerLyzer™ UltraClean® Tissue RNA Isolation Kit is designed to provide efficient purification of total RNA from a variety of mammalian tissues or cultured cells.
Prepared fresh or frozen tissues are homogenized using a bead based homogenizer such as the PowerLyzer™ 24, in the presence of lysis solutions. The RNA is then bound to a silica spin filter, washed and eluted into certified RNase-free water (provided). Kit includes all RNase-free reagents ready to use and all required spin filters and tubes. RNA is of high quality and is ready for any downstream applications such as RT or RT-PCR.
Requires our PowerLyzer™ 24 for maximum efficiency.
Sample Kit
Click to try a sample.
Click over image to enlarge
MSDS
Protocol
Specifications
View the full PowerLyzer™ UltraClean®
Tissue & Cells RNA Application Note
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Based Homogenization |
| Starting Amount | 10 to 30 mg |
| Binding Capacity | Up to 60 µg per filter |
| Throughput | 1 - 24 samples |
| Time | 20 minutes |
Storage Conditions
Store at room temperature for up to 2 years.Kit Components
| Component | 15000-50 |
|---|---|
| Solution TR1 Solution TR2 Solution TR3 Solution TR4 Solution TR5 Spin Filters in Tubes Collection Tubes PowerLyzer™Ceramic Bead Tubes |
1 x 33 ml 1 x 33 ml 1 x 28 ml 2 x 28 ml 1 x 8 ml 50 150 50 |
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