UltraClean® Tissue & Cells DNA Isolation Kit

Overview

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Features

- Rapidly isolate DNA from animal tissues or cultured cells in less than 20 minutes.

- Yields high quality DNA up to 50 kb plus

- DNA is ready for PCR, restriction digests, and all downstream applications.

- Novel bead beating method for hands-off homogenization.

- Safe procedure avoids the use of hazardous organic solvents.

- Lyophilized Proteinase K has been included in the kit for processing tough tissue samples.

Description

The UltraClean^® Tissue & Cells DNA Isolation Kit is ideal for isolating genomic DNA from cultured cells or animal tissues, including rodent tails. Isolated DNA is high quality and ready for PCR, restriction digests, and other downstream applications. The UltraClean^® Tissue & Cells DNA Isolation Kit is safe and user-friendly because it avoids the use of organic solvents like phenol and chloroform.

The UltraClean^® Tissue & Cells DNA Isolation Kit is designed for isolating DNA from 1-25 mg tissue samples or up to 5 x 10⁶ cells.

Fresh or frozen tissue samples are homogenized using bead beating technology to lyse the cells. Lysates are loaded onto a silica spin filter. DNA binds to the silica spin filter while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed by a wash step. Pure DNA is then eluted into Tris buffer.This kit requires a microcentrifuge, vortex and our Vortex Adapter.

This kit contains an optional step for tough tissue samples such as bone, hair and mouse tails. Lyophilized Proteinase K has been included in this kit for processing these tissue samples.

Sample Kit

Click to try a sample.

Research and Development

 

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Genomic DNA isolated from mouse tissues using the UltraClean^® Tissue & Cells DNA Isolation Kit was digested with EcoR I and displayed on a 1.2% TAE agarose gel. Lanes 1,3,5,7,9: undigested DNA. Lanes: 2,4,6,8,10: Digested DNA.

Lanes 1,2: Brain Lanes 3,4: Heart Lanes 5,6: Kidney

Lanes 7,8: Liver Lanes 9,10: Spleen Lane 11: 1 kb marker

Comparison of time required to isolate genomic DNA from animal tissue using different Tissue DNA Isolation Systems. Bars indicate time required to perform procedure without tissue lysis (blue) and with tissue lysis steps included (red).

MSDS

12334

Protocol

12334

Specifications

Storage Conditions

Publications

• Casper, R.M., Jarman, S.N., Deagle, B.E., Gales, N.J., Hindell, M.A. Detecting Prey From DNA In Predator Scats: A Comparison With Morphological Analysis, Using Arctocephalus Seals Fed a Known Diet. Journal of Experimental Marine BIology and Ecology. 347(1-2): 144 (2007).
• Casper, R.M., Jarman, S.N., Gales, N.J., Hindell, M.A. Combining DNA and Morphological Analyses Of Faecal Samples Improves Insight Into Trophic Interactions: A Case Study Using A Generalist Predator. Marine Biology. 152(4): 815-825 (2007).
• Estonba, A., Solís, A., Iriondo, M., Sanz-Martín, M.J., Pérez-Suárez, G., Markov, G., Palacios, F. The Genetic Distinctiveness Of The Three Iberian Hare Species: Lepus europaeus, L. granatensis, and L. castroviejoi. Mammalian Biology. 71(1): 52-59 (2006).

Kit Components