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UltraClean® -htp 96 Well Tissue DNA Isolation Kit
Overview
For high throughput isolation of genomic DNA from animal tissues and cellsOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 12996-4 | $932.00 |
|
| 12 x 96 preps | 12996-12 | $2,599.00 |
|
Features
- Process 96 animal tissue samples in less than 45 minutes
- Yields high quality DNA, up to 50kb plus
- DNA is ready for PCR, restriction digests, and all downstream applications
- Novel bead beating method for hands-off homogenization. DNA isolated using silica spin filter plate format
- Safe procedure avoids the use of hazardous organic solvents
Description
The UltraClean-htp® 96 Well Tissue DNA Isolation Kit is designed for rapid, high throughput DNA isolation from fresh or frozen animal tissues, cells and rodent tails.
Fresh or frozen tissue samples are homogenized by bead beating in a deep well plate containing beads, bead solution and lysis solution. These plates are processed on a high velocity 96 Well Plate Shaker. This hands-off homogenization is optimized for even the toughest tissues including rodent tails. Cells are lysed and DNA is bound to a silica membrane spin filter plate. The DNA is washed and then eluted into certified DNA-free Tris buffer.
This efficient procedure and convenient 96 well format eliminate the need for organic extraction, labeling of individual tubes, one-at-a-time pipetting, and DNA resuspension so that downstream appplications such as PCR can be completed on the same day.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Plate |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 40 µg per well |
| Throughput | 96 samples |
| Time | 45 minutes |
|
Storage Conditions
Store at room temperature for up to 2 years.Kit Components
| Component | 12996-4 | 12996-12 |
|---|---|---|
| Bead Plates Proteinase K Solution TD1 Solution TD2 Solution TD3 Spin Plates 0.5 ml Collection Plates Microplates Centrifuge Tape Elution Sealing Mat |
4 1 x 8.5 ml 1 x 300 ml 1 x 170 ml 1 x 43 ml 4 4 4 28 4 |
12 1 x 25.5 ml 1 x 900 ml 1 x 500 ml 1 x 128 ml 12 12 12 84 12 |
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