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BiOstic® FFPE Tissue DNA Isolation Kit
Overview
For the extraction of genomic DNA from FFPE tissue samplesOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12250-50 | $153.00 |
|
Features
- Faster than solvent wax removal methods
- Higher yields of DNA due to fewer steps and less chance of sample loss
- More sensitive results and lower Ct values in real-time PCR due to higher DNA purity (contaminant-free)
- Safe method avoids xylene and other toxic solvents
Description
The BiOstic® FFPE Tissue DNA Isolation Kit is designed to extract and purify genomic DNA from tissues fixed with formalin and embedded in paraffin.
Using a novel method, the BiOstic® FFPE Tissue DNA Isolation Kit directly melts wax away from tissue without the use of solvents such as xylene to deparaffinize the tissue. Higher DNA yield is achieved as a result of this gentle method. Also, the recovered DNA is more pure and free from contaminants that can have an adverse affect on detection sensitivity and amplification efficiency. Genomic DNA extracted with the BiOstic® FFPE Tissue DNA Isolation Kit can be used in real-time PCR, SNP genotyping and other genetic analysis methods.
May be used in combination with the BiOstic® Paraffin Removal Reagent. Protocol included in the BiOstic® FFPE Tissue DNA Isolation Kit manual.
The BiOstic® FFPE Tissue DNA Isolation Kit is sold for research purposes only.
Sample Kit
Click to try a sample.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Direct melting of FFPE tissues |
| Sample Size | One slice up to 15 mg of tissue |
| Binding Capacity | Up to 20 µg per spin filter |
| DNA Size Range | Up to 12 kb |
| Throughput | 1-24 samples |
| Time |
2 hrs for melting and de-cross-linking, 20 minutes for spin prep |
| Equipment Required | Vortex, centrifuge, and water bath or heat block |
Storage Conditions
Proteinase K is stable at room temperature. For prolonged storage, place the Solution FP3 (Proteinase K) at 4oC. Store all other reagents and kit components at room temperature (15-30oC) up to 2 years.
Publications
- Shawn E. Yost, Erin N. Smith, Richard B. Schwab, Lei Bao, HyunChul Jung, Xiaoyun Wang, Emile Voest, John P. Pierce, Karen Messer, Barbara A. Parker, Olivier Harismendy, and Kelly A. Frazer. Identification of high-confidence somatic mutations in whole genome sequence of formalin-fixed breast cancer specimens. Nucleic Acids Res. 1–12 (2012).
Kit Components
| Component | 12231-50 |
|---|---|
|
Solution FP1 Solution FP2 Solution FP3 (Proteinase K 20mg/ml) Solution FP4 Solution FP5 (100% ethanol) Solution FP6 Solution FP7 Solution FP8 Spin Filters 2 ml Collection Tubes |
11 ml 1.2 ml 1.2 ml 11 ml 11 ml 28 ml 28 ml 11 ml 50 150 |
