The RNA PowerSoil® Total RNA Isolation Kit utilizes our novel and proprietary Inhibitor Removal Technology® (IRT) for isolating total RNA from up to 2 grams of soil. RT-PCR and PCR inhibiting substances, including humic substances and the brown color often associated with reverse transcription and PCR inhibition are completely removed. The kit is intended for use with all common soil types, but in particular, with samples that typically contain a high humic substance content, including compost, sediment and manure.
The kit offers an easy-to-follow procedure. Environmental samples are added to a bead beating tube with a kit-supplied buffer for rapid and thorough homogenization. Total RNA is captured on a proprietary matrix in a column format, washed and eluted. RNA is RT-PCR and PCR ready without further handling or purification.
IMPORTANT NOTE: This kit requires the addition of phenol:chloroform (see protocol)
Need DNase? Try our RNA PowerSoil® + RTS DNase Bundle* and save on the purchase of RNA PowerSoil and The RTS DNase Kit!
The RTS DNase™ Kit is used for the removal of genomic DNA contamination in RNA preparations. RTS DNase will remove up to 30 µg of DNA in 20 minutes using 10 units (1 µl) of enzyme. The enzyme is stable for up to 6 months at room temperature with no loss of activity and for 2 years at 4°C without loss of activity. The RTS DNase™ Kit also contains a novel and highly specific resin which is used to bind and remove the RTS DNase enzyme and divalent cations from the reaction, eliminating the need for heat or EDTA inactivation of the DNase. The RNA is ready to use immediately after resin treatment. Learn More
Try our RNA PowerSoil® DNA Elution Accessory Kit . Now you can co-isolate DNA from the same starting sample!
*US direct orders only, through September 30, 2014
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High RNA Yield and Reliable RT-PCR Results
Total RNA was isolated from 8 different soil samples using the RNA PowerSoil® Total RNA Isolation Kit (lanes 1-8). RNA was displayed (A) on a 1% x TAE agarose gel (2 µg per lane). RT-PCR analysis with primers representing the Streptomyces spp. (B) and Bacillus spp. (C) was performed using 1 µl of the undiluted RNA and analyzed on a 1% TAE agarose gel and stained with ethidium bromide. N = negative control. P = positive control. Soil types and amounts are identified below. Positive RT-PCR amplification was observed with 100% of the samples.
|Sample Lane||Type||Amount processed (grams)|
|3||Plant root rhizosphere||2|
|4||Carlsbad lagoon sediment||2|
|Format||Proprietary Column Technology|
|Starting Amount||2 g|
|Equipment Required||Vortex and Vortex Adapter|