RNA PowerSoil® Total RNA Isolation Kit

Overview

Related Products

Features

- Processes all soil types including difficult environmental samples

- Successful RT-PCR with all tested samples

- Purifies RNA from up to 2 grams of soil in less than 2.5 hours

- Yields high quality RNA free of RT-PCR inhibitors and humic substances

- Eliminates visible brown coloration

Description

In less than 2.5 hours, the RNA PowerSoil^® Total RNA Isolation Kit utilizes our novel and proprietary Inhibitor Removal Technology^® (IRT) for isolating total RNA from up to 2 grams of soil. RT-PCR and PCR inhibiting substances, including humic substances and the brown color often associated with reverse transcription and PCR inhibition are completely removed. The kit is intended for use with all common soil types, but in particular, with environmental samples that typically contain high humic substance content, including compost, sediment and manure. RT-PCR was used to analyze undiluted total RNA purified using the RNA PowerSoil^® Total RNA Isolation Kit. Primers were specific for Bacillus and Streptomyces spp. (see A, B C).

The kit offers a simple and easy-to-follow procedure. Environmental samples are added to a bead beating tube with a kit-supplied buffer for rapid and thorough homogenization. Total RNA is captured on a proprietary matrix in a column format, washed and eluted. RNA is RT-PCR and PCR ready without further handling or purification.

IMPORTANT NOTE: This kit requires the addition of phenol:chloroform (see protocol)

Try our new RNA PowerSoil^® DNA Elution Accessory Kit . Now you can co-isolate DNA from the same starting sample!

Sample Kit

Click to try a sample.

Research and Development

RNA PowerSoil^® Total RNA Isolation Kit - More Effective for Community Analysis using RT-PCR

High Yield and Reliable RT-PCR Results

Total RNA was isolated from 8 different soil samples using the RNA PowerSoil^® Total RNA Isolation Kit (lanes 1-8). RNA was displayed (A) on a 1% x TAE agarose gel (2 µg per lane). RT-PCR analysis with primers representing the Streptomyces spp. (B) and Bacillus spp. (C) was performed using 1 µl of the undiluted RNA and analyzed on a 1% TAE agarose gel and stained with ethidium bromide. N = negative control. P = positive control. Soil types and amounts are identified below.
 

Positive RT-PCR amplification using the RNA PowerSoil^® Total RNA Isolation Kit was observed with 100% of the samples.

MSDS

12866

Protocol

12866

Specifications

Storage Conditions

Publications

• Accinelli, C., Abbas, H.K., Zablotowicz, R.M., Wilkinson, J.R. Aspergillus flavus Aflatoxin Occurrence and Expression Of Aflatoxin Biosynthesis Genes In Soil. Canadian Journal of Microbiology. 54(5): 371-379 (2008).
• Lee, P.K., Macbeth, T.W., Sorenson, K.S. Jr., Deeb, R.A., Alvarez-Cohen, L. Quantifying Genes and Transcripts To Assess The In Situ Physiology Of "Dehalococcoides" spp. In A Trichloroethene-Contaminated Groundwater Site. Applied and Environmental Microbiology. 74(9): 2728-2739 (2008).
• McGrath, K.C., Thomas-Hall, S.R., Cheng, C.T., Leo, L., ALexa, A., Schmidt, S., and Schenk, P.M. Isolation and Analysis of mRNA from Environmental Microbial Communities. Journal of Microbiological Methods. 75: 712-176 (2008).

Kit Components