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UltraClean® Soil DNA Isolation Kit
Overview
For isolating genomic DNA from routine soil and fecal samplesOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12800-50 | $181.00 |
|
| 100 preps | 12800-100 | $360.00 |
|
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UltraClean® Fecal DNA Isolation Kit
UltraClean® Mega Soil DNA Isolation Kit
Features
- Established method selectively removes most PCR inhibitors
- Rapidly purify DNA from up to one gram of soil in 30 minutes
- Yields high quality PCR ready DNA up to 50 kb plus
- Includes a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR
- Efficiently lyses spores and fungi; also compatible with fecal samples
Description
The UltraClean® Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors.
Soil samples are added to a bead beating tube containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer.
This is our original DNA kit for soil - for more challenging soil samples we recommend the PowerSoil® DNA Isolation Kit
NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System!
Sample Kit
Click to try a sample.
Research and Development
Purification of DNA from various soils using
the UltraClean® Soil DNA Isolation Kit
| M | 1 | 2 | 3 | 4 |
- Lane 1: Forest soil
Lane 2: Bog soil
Lane 3: Pristine sand
Lane 4: Farm soil
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
- Lane 1: Marker
Lane 2: Negative control
Lanes 3-8: Six different soil types
Learn more about the applications of the UltraClean® Soil DNA Isolation Kit in this poster presented at the American Society for Microbiology Meeting.
Testing the UltraClean® Soil DNA Isolation Kit
on a Diverse Range of Soils
by PCR Amplification of 16S rDNA.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per filter |
| Throughput | 1 - 24 samples |
| Time | 30 minutes |
| Equipment Required | Vortex Adapter |
with these Soil Types |
|
|---|---|
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Publications
- Andreote, F., Carneiro, R., Salles, J., Marcon, J., Labate, C., Azevedo, J., and Araujo, W. Culture-Independent Assessment of Rhizobiales-Related Alphaproteobacteria and the Diversity of Methylobacterium in the Rhizosphere and Rhizoplane of Transgenic Eucalyptus. Microbial Ecology. 57: 82-93 (2009).
- Baniulyte, D., Favila, E., and Kelly, J.Shifts in Microbial Community Composition Following Surface Application of Dredged River Sediment. Microbial Ecology. 57: 160-169 (2009).
- Barkovskii, A., Fukui, H., Leisen, J., Kim, S., Marsh, T., and Khijniak, A. Rearrangement of Bacterial Community Structure During Peat Diagenesis. Soil Biology and Biochemistry. 41: 135-143 (2009).
Customer Comments
I consulted a soil microbiologist for advice on a protocol for rapid DNA extraction from soil samples. He recommended the MO BIO UltraClean® Soil DNA Isolation Kit. My research has involved extensive soil sampling. The soil is analyzed for the presence of Streptomyces scabies, causal agent of potato scab, which is detectable by PCR. I have used the kit to process samples and have been extremely pleased with the results of not only getting consistent amounts of DNA, but also DNA that is high quality for PCR. I will continue using this kit because it is consistent and extremely quick. No other kit I have used is even half as fast.
Elise C. Poole
Michigan State University
Dept. of Botany and Plant Pathology
Kit Components
| Component | 12800-50 | 12800-100 |
|---|---|---|
| Bead Tubes Solution S1 IRS Solution Solution S2 Solution S3 Solution S4 Solution S5 Spin Filters In Tubes Collection Tubes |
50 1 x 3.3 ml 1 x 11 ml 1 x 14 ml 1 x 72 ml 1 x 16.5 ml 1 x 3 ml 50 150 |
100 1 x 6.6 ml 1 x 22 ml 1 x 27.5 ml 1 x 143 ml 1 x 30 ml 1 x 6 ml 100 300 |
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