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UltraClean®-htp 96 Well Soil DNA Isolation Kit
Overview
For high throughput isolation of DNA from up to 384 soil samples in less than one dayOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 12896-4 | $1,736.00 |
|
| 12 x 96 preps | 12896-12 | $4,687.00 |
|
Features
- Processes up to 384 soil samples in less than one day.
- Yields high quality PCR ready DNA up to 50kb plus
- Includes Inhibitor Removal Technology® to eliminate humic acid inbitors of PCR
- Efficiently lyses spores and fungi; also compatible with fecal samples
- Flexible format with protocols available for centrifiguation or vacuum
Description
The UltraClean®-htp 96 Well Soil DNA Isolation Kit provides the UltraClean® soil technology in a high-throughput 96 well format. This kit requires a plate centrifuge, a 96 well plate shaker and optional Vacuum Manifold.
The UltraClean®-htp 96 Well Soil DNA Isolation Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors.
Soil samples are added to a 96 well block containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a 96 well plate shaker. From the lysed cells, the released DNA is bound to a silica spin plate. The plate is washed, and the DNA is recovered in certified DNA-free Tris buffer.
Research and Development
Consistent Yields and
Well-to-Well Reproducibility obtained with
UltraClean®-htp 96 Well Soil DNA Isolation Kit
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Genomic DNA was isolated from different soil samples.
Analysis displayed on 1.2% TAE agarose gel.
Learn more about the applications of the
UltraClean®-htp 96 Well Soil DNA Isolation Kit in this article.
Novel High Throughput Method for Studying
Microbial Communities and Genes in Soil
Download Here (pdf 1.8MB)
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin FilterPlate |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per well |
| Sample Size | 250 mg |
| Throughput | 96 - 384 samples |
| Time | 4 x 96 samples in <1 day |
Equipment Required |
Plate centrifuge, 96 well plate shaker, UltraVac™ Manifold* |
* Optional
Obtained With These Soil Types |
|
Storage Conditions
Store at room temperature for up to 2 years.Publications
- Venugopal, R.J., Brolaski, M.N. Novel High Throughput Method For Studying Microbial Communities and Genes In Soil. Bioscience Technology. 28(5): 48-49 (2003).
Kit Components
| Component | 12896-4 | 12896-12 |
|---|---|---|
| Bead Plates Bead Solution Solution S1 IRS Solution Solution S2 Solution S3 Solution S4 Solution S5 Spin Plates 2 ml Collection Plates 1 ml Collection Plates 0.5 ml Collection Plates Microplates Centrifuge Tape Sealing Tape Elution Sealing Mat |
4 1 x 233 ml 1 x 26 ml 1 x 85 ml 1 x 106 ml 1 x 550 ml 4 x 30 ml 1 x 43 ml 4 8 8 4 4 24 8 4 |
12 1 x 699 ml 1 x 78 ml 1 x 255 ml 1 x 318 ml 1 x 1650 ml 12 x 30 ml 1 x 129 ml 12 24 24 12 12 72 24 12 |
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