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PowerSoil®-htp 96 Well Soil DNA Isolation Kit

Overview

For high throughput isolation of DNA from up to 384 soil samples in less than one day
Order
Description Catalog # Price
4 x 96 preps 12955-4 $1,916.00    
12 x 96 preps 12955-12 $5,171.00    

Features

  • Next generation method improves PCR inhibitor removal efficiency
  • Processes the largest variety of soil types, including difficult environmental samples
  • Patented Inhibitor Removal Technology® removes 100% of humic substances and PCR inhibitors
  • Effective purification of DNA from 250 mg of soil
  • Simultaneously process 4 plates with 96 samples each
  • Flexible format - protocols available for centrifugation or vacuum formats

Description

The PowerSoil®-htp 96 Soil DNA Isolation Kit is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples in a 96 well format for high throughput. The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, marine sediment, and manure among others. The isolated DNA has a high level of purity allowing for more successful PCR amplification of DNA from organisms within the sample. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces) and nematodes.

The PowerSoil®-htp 96 Well Soil DNA Kit distinguishes itself from MO BIO's UltraClean® Soil DNA Isolation Kit with a patented Inhibitor Removal Technology® (IRT), which removes humic substances/brown color. This new procedure is effective at removing PCR inhibitors from even the most difficult soil types.

Environmental samples are added to a 96 well bead beating plate for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a 96 well silica membrane in a spin column format. DNA is then washed and eluted from the spin column. DNA is ready for PCR analysis and other downstream applications.

PowerSoil®-htp 96 Well Soil DNA Isolation Kit
Click over image to enlarge

MSDS

12955

Protocol

12955

Specifications

View the full PowerSoil®-htp 96 Well 
DNA Application Note

PowerSoil-htp 96 Well DNA Application Note

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 20 µg per well
Sample Size 250 mg
Throughput 96 - 384 samples
Time 384 samples in less than one day
Equipment Required Plate centrifuge and plate shaker

Kit Components

Component 12955-4 12955-12
Bead Plate
Bead Solution
Solution C1
Solution C2
Solution C3
Solution C4
Solution C5-D
Solution C6
High Spin Plates
2 ml Collection Plates
1 ml Collection Plates
0.5 ml Collection Plates
Microplates
Centrifuge Tape
Sealing Tape
Elution Sealing Mats
4
340 ml
45 ml
128 ml
106 ml
550 ml
120 ml
66 ml
4
8
16
4
4
24
16
4
12
1020 ml
135 ml
385 ml
320 ml
2 x 825 ml
360 ml
200 ml
12
24
48
12
12
72
48
12