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PowerSoil®-htp 96 Well Soil DNA Isolation Kit
Overview
For high throughput isolation of DNA from up to 384 soil samples in less than one dayOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 12955-4 | $1,732.00 |
|
| 12 x 96 preps | 12955-12 | $4,676.00 |
|
Features
- Next generation method improves PCR inhibitor removal efficiency
- Processes the largest variety of soil types, including difficult environmental samples
- Patented Inhibitor Removal Technology® removes 100% of humic substances and PCR inhibitors
- Effective purification of DNA from 250 mg of soil
- Simultaneously process 4 plates with 96 samples each
- Flexible format - protocols available for centrifugation or vacuum formats
Description
The PowerSoil®-htp 96 Soil DNA Isolation Kit is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples in a 96 well format for high throughput. The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, marine sediment, and manure among others. The isolated DNA has a high level of purity allowing for more successful PCR amplification of DNA from organisms within the sample. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces) and nematodes.
The PowerSoil®-htp 96 Well Soil DNA Kit distinguishes itself from MO BIO's UltraClean® Soil DNA Isolation Kit with a patented Inhibitor Removal Technology® (IRT), which removes humic substances/brown color. This new procedure is effective at removing PCR inhibitors from even the most difficult soil types.
Environmental samples are added to a 96 well bead beating plate for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a 96 well silica membrane in a spin column format. DNA is then washed and eluted from the spin column. DNA is ready for PCR analysis and other downstream applications.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per well |
| Sample Size | 250 mg |
| Throughput | 96 - 384 samples |
| Time | 384 samples in less than one day |
| Equipment Required | Plate centrifuge and plate shaker |
Kit Components
| Component | 12955-4 | 12955-12 |
|---|---|---|
| Bead Plate Bead Solution Solution C1 Solution C2 Solution C3 Solution C4 Solution C5-D Solution C6 High Spin Plates 2 ml Collection Plates 1 ml Collection Plates 0.5 ml Collection Plates Microplates Centrifuge Tape Sealing Tape Elution Sealing Mats |
4 340 ml 45 ml 128 ml 106 ml 550 ml 120 ml 66 ml 4 8 16 4 4 24 16 4 |
12 1020 ml 135 ml 385 ml 320 ml 2 x 825 ml 360 ml 200 ml 12 24 48 12 12 72 48 12 |
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