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PowerSoil®-htp 96 Well Soil DNA Isolation Kit
Overview
For high throughput isolation of DNA from up to 384 soil samples in less than one dayOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 12955-4 | Call for price | |
| 12 x 96 preps | 12955-12 | Call for price | |
Features
- Next generation method improves PCR inhibitor removal efficiency
- Processes the largest variety of soil types, including difficult environmental samples
- Patented Inhibitor Removal Technology® removes 100% of humic substances and PCR inhibitors
- Effective purification of DNA from 250 mg of soil
- Simultaneously process 4 plates with 96 samples each
- Flexible format - protocols available for centrifugation or vacuum formats
Description
The PowerSoil®-htp 96 Soil DNA Isolation Kit is comprised of a novel and proprietary method for isolating genomic DNA from environmental samples in a 96 well format for high throughput. The kit is intended for use with environmental samples containing a high humic acid content including difficult soil types such as compost, marine sediment, and manure among others. The isolated DNA has a high level of purity allowing for more successful PCR amplification of DNA from organisms within the sample. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces) and nematodes.
The PowerSoil®-htp 96 Well Soil DNA Kit distinguishes itself from MO BIO's UltraClean® Soil DNA Isolation Kit with a patented Inhibitor Removal Technology® (IRT), which removes humic substances/brown color. This new procedure is effective at removing PCR inhibitors from even the most difficult soil types.
Environmental samples are added to a 96 well bead beating plate for rapid and thorough homogenization. Cell lysis occurs by mechanical and chemical methods. Total genomic DNA is captured on a 96 well silica membrane in a spin column format. DNA is then washed and eluted from the spin column. DNA is ready for PCR analysis and other downstream applications.
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MSDS
Protocol
Specifications
View the full PowerSoil®-htp 96 Well
DNA Application Note
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per well |
| Sample Size | 250 mg |
| Throughput | 96 - 384 samples |
| Time | 384 samples in less than one day |
| Equipment Required | Plate centrifuge and plate shaker |
Kit Components
| Component | 12955-4 | 12955-12 |
|---|---|---|
| Bead Plate Bead Solution Solution C1 Solution C2 Solution C3 Solution C4 Solution C5-D Solution C6 High Spin Plates 2 ml Collection Plates 1 ml Collection Plates 0.5 ml Collection Plates Microplates Centrifuge Tape Sealing Tape Elution Sealing Mats |
4 340 ml 45 ml 128 ml 106 ml 550 ml 120 ml 66 ml 4 8 16 4 4 24 16 4 |
12 1020 ml 135 ml 385 ml 320 ml 2 x 825 ml 360 ml 200 ml 12 24 48 12 12 72 48 12 |
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