PowerSoil® DNA Isolation Kit

Overview

Related Products

Features

• Isolate high quality, inhibitor-free DNA from soil, stool and environmental samples

• Patented Inhibitor Removal Technology eliminates humic substances and other PCR inhibitors

• Rapid protocol enables purification of DNA from 250 mg soil in 30 minutes

Description

The PowerSoil® DNA Isolation Kit uses patented Inhibitor Removal Technology® to isolate microbial genomic DNA from all soil types and environmental samples, as well as fecal, stool and biosolid samples. The isolated DNA has the highest level of purity, enabling successful PCR and qPCR amplification of organisms from the sample. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes. The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that eliminates PCR inhibitors from even the most difficult soil types, enabling samples to be used for the most demanding downstream applications, including PCR, qPCR and next generation sequencing. 

Sample Kit

Click to try a sample.

Research and Development

The PowerSoil^® DNA Isolation Kit is More Effective for Community Analysis by PCR 
 

	High Yield, Intact Genomic DNA
MO BIO PowerSoil® Supplier A Supplier B
	M   1   2   3   4   5   6   7   8   9

Total Genomic DNA was isolated from nine different sample types using the MO BIO PowerSoil® DNA Isolation Kit and soil DNA isolation kits from two other suppliers. All isolations were performed following the manufacturerís protocols. Eluted genomic DNA was displayed on a 0.8% TAE agarose gel (15 µl per lane).

	Confidence in Your PCR Analysis
MO BIO PowerSoil® Supplier A Supplier B
	M   1   2   3   4   5   6   7   8   9

PCR analysis using eubacterial primers was performed on 1 µl of the undiluted DNA elute. PCR products were displayed on a 0.8% TAE agarose gel. Lane M is DNA Marker.

Only DNA isolated with PowerSoil^® yielded a PCR product for Landfill samples (lanes 1 and 2), Lake Sediment (lane 6) and Mulch Topsoil (lane 9). DNA isolated using other supplier kits failed to amplify products for these samples, most likely due to high levels of humic acid substances remaining in the final DNA eluate.

Positive PCR amplification using PowerSoil^® was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with supplier kits tested.

MSDS

12888

Protocol

12888

Specifications

Visual Protocol

Storage Conditions

Publications

• Geml, J., Laursen, G. A., Herriott, I. C., McFarland, J. M., Booth, M.G., Lennon, N., Chad Nusbaum, H. and Lee Taylor, D. Phylogenetic and ecological analyses of soil and sporocarp DNA sequences reveal high diversity and strong habitat partiimages/custom/file/pdf/Phylogenetic-and-ecological-analyses-of-soil.pdftioning in the boreal ectomycorrhizal genus Russula(Russulales; Basidiomycota). New Phytologist, 187: 494–507. doi: 10.1111/j.1469-8137.2010.03283.
• Makhalanyane TP, Valverde A, Lacap DC, Pointing SB, Tuffin MI, Cowan DA. Evidence of species recruitment and development of hot desert hypolithic communities. Environmental Microbiology Reports (2013) 5: 219-224.
• Subramanya Rao, Kevin D. Hyde, Stephen B. Pointing. Comparison of DNA and RNA, and Cultivation Approaches for the Recovery of Terrestrial and Aquatic Fungi from Environmental Samples. Curr Microbiol (2013) 66:185–191

Kit Components