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PowerSoil® DNA Isolation Kit

Overview

Isolate microbial genomic DNA from soil, stool and other environmental samples.
Order
Description Catalog # Price
50 Preps 12888-50 $257.00    
100 preps 12888-100 $494.00    

Features

  • Optimized lysis for tough samples – Isolate pure DNA from soil, stool, and other environmental samples
  • Inhibitor Removal Technology® - Eliminates 100% of humic substances and other PCR inhibitors, for DNA that is ready to use in downstream applications
  • Rapid protocol - Enables isolation of high quality, pure DNA from 250 mg samples in just 30 minutes

Description

The PowerSoil® DNA Isolation Kit uses patented Inhibitor Removal Technology® to isolate microbial genomic DNA from all soil types and environmental samples, as well as fecal, stool and biosolid samples. The isolated DNA has the highest level of purity, enabling successful PCR and qPCR amplification of organisms from the sample. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes. The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that eliminates PCR inhibitors from even the most difficult soil types, enabling samples to be used for the most demanding downstream applications, including PCR, qPCR and next generation sequencing. 

CUSTOMER REVIEW

“I used the kit to extract DNA from grape phylloxera in a soil sample that had high levels of composted material / humic acids. It worked very well compared to the Qiagen Stool kit and the final product from the extraction went through PCR without any problems. Thanks for making my life easier.”

- Dave, Washington Government, Plant Pathology Lab

Sample Kit

Click to try a sample.

Research and Development

The PowerSoil® DNA Isolation Kit is More Effective for Community Analysis by PCR 
 

  High Yield, Intact Genomic DNA
MO BIO
PowerSoil®




Supplier A



Supplier B
 
     M   1   2   3   4   5   6   7   8   9

Total Genomic DNA was isolated from nine different sample types using the MO BIO PowerSoil® DNA Isolation Kit and soil DNA isolation kits from two other suppliers. All isolations were performed following the manufacturerís protocols. Eluted genomic DNA was displayed on a 0.8% TAE agarose gel (15 µl per lane).

  Confidence in Your PCR Analysis
MO BIO
PowerSoil®




Supplier A



Supplier B
   
     M   1   2   3   4   5   6   7   8   9

PCR analysis using eubacterial primers was performed on 1 µl of the undiluted DNA elute. PCR products were displayed on a 0.8% TAE agarose gel. Lane M is DNA Marker.

Lane 1 - Landfill 0-3 inches       Lane 4 - Coffee Compost       Lane 7 - Mud Sediment
Lane 2 - Landfill 3-6 inches       Lane 5 - Marine Sediment       Lane 8 - Horse Manure
Lane 3 - Late-stage Compost       Lane 6 - Lake Sediment       Lane 9 - Mulch Topsoil

Only DNA isolated with PowerSoil® yielded a PCR product for Landfill samples (lanes 1 and 2), Lake Sediment (lane 6) and Mulch Topsoil (lane 9). DNA isolated using other supplier kits failed to amplify products for these samples, most likely due to high levels of humic acid substances remaining in the final DNA eluate.

Positive PCR amplification using PowerSoil® was observed with 100% of the samples. PCR analysis resulted in only 44% positive amplification with supplier kits tested.

PowerSoil® DNA Isolation Kit
Click over image to enlarge

MSDS

12888

Protocol

12888

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 20 µg per filter
Sample Size 250 mg
Throughput 1 - 24 samples
Time 30 minutes
Equipment Required Vortex and Vortex Adapter

Visual Protocol

Storage Conditions

Store at room temperature for up to 2 years.

Publications

Kit Components

Component 12888-50 12888-100
PowerBead Tubes
Solution C1
Solution C2
Solution C3
Solution C4
Solution C5
Solution C6
Spin Filters In Tubes
Collection Tubes
50
1 x 3.3 ml
1 x 14 ml
1 x 11 ml
1 x 72 ml
1 x 27.5 ml
1 x 6 ml
50
200
100
1 x 6.6 ml
1 x 28 ml
1 x 22 ml
1 x 144 ml
1 x 55 ml
1 x 12 ml
100
400