PowerMax® Soil DNA Isolation Kit

Overview

Related Products

Features

• Purifies DNA from 10 g soil in 30 minutes - means NO time spent on multiple small sample processing

•  Increases detection sensitivity on samples with a low microbial load

•  Provides high quality DNA that is free of PCR inhibitors including humic substances

• Purified samples are suitable for PCR and qPCR

Description

The PowerMax^® Soil DNA Isolation Kit is our next-generation kit designed for processing large quantities of any soil or environmental sample with high or low microbial load. Based on reagents from the PowerSoil^® DNA Isolation Kit, the PowerMax^® Soil DNA Isolation Kit uses our novel, patented Inhibitor Removal Technology^® to remove PCR inhibiting compounds, including humic substances, and the brown color often associated with soil DNA. The kit is ideal for environmental samples that contain high humic substance content, including compost, sediment and manure. PCR analysis has been used to amplify DNA purified from microorganisms including Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces).

The PowerMax^® Soil DNA Isolation Kit increases your ability to study microbial diversity in even the most difficult sample types, such as those which contain high humic levels or low microbial load. Our 50 ml Vortex Adapter for cell lysis and a centrifuge capable of spinning 50 ml tubes are required for maximum performance.

Sample Kit

Click to try a sample.

Research and Development

High Yield, Intact Genomic DNA

Total genomic DNA was isolated from 8 different soil samples using the PowerMax® Soil DNA Isolation Kit. 0.75 µg of each isolated DNA was analyzed on 1 % TAE agarose gel and ethidium stained. M = Marker DNA. Soil types are identified in the table below. One microliter of total genomic DNA isolated using the PowerMax® Soil DNA Isolation Kit was PCR amplified using primers to the Bacillus spp. Amplified DNA was analyzed on 0.8% TAE agarose and ethidium stained. The amount of PCR fragment produced in each sample is representative of the relative amount of the template sequence in the purified DNA. Different soil sources will have different types and amount of organisms providing genomic DNA. N = Negative control lacking template. P = Positive control template. Soil types and amount are identified in the table below.   One microliter of total genomic DNA isolated using the PowerMax® Soil DNA Isolation Kit was PCR amplified using primers to the Streptococcus spp. DNA was analyzed on 1 % TAE agarose and ethidium stained. The amount of PCR fragment produced in each sample is representative of the relative amount of template sequence in the purified. Different soil sources will have different types and amount of organisms providing genomic DNA. N = Negative control lacking template. P = Positive control template. Soil types and amount are identified below. Sample Lane Type Amount processed (grams) 1 Iowa corn field 10 2 So. California strawberry field 10 3 Cardiff estuary sediment 10 4 Carlsbad lagoon sediment 10 5 Home compost 5 6 San Diego City compost 5 7 Commercial potting mixture 2.5 8 Commercial peat moss 2.5

MSDS

12988

Protocol

12988

Specifications

Storage Conditions

Publications

• Holly H Ganz, Ulas Karaoz, Wayne M Getz, Wilferd Versfeld, And Eoin, L Brodie. Diversity and structure of soil bacterial communities associated with vultures in an African savanna. Ecosphere. 06/2012; 3(6):1-18.
• Nagissa Mahmoudi, Greg F. Slater, and Roberta R. Fulthorpe. Comparison of commercial DNA extraction kits for isolation and purification of bacterial and eukaryotic DNA from PAH-contaminated soil. Can J Microbiol. 2011 Aug;57(8):623-8. Epub 2011 Aug 4.
• Mountfort, D.; Smith, K. F.; Kirs, M.; Kuhajek, J.; Adamson, J. E.; Institute, S. A. W. C.; Bag, P.; Nelson; Zealand, N. Development of single and multispecies detection methods for the surveillance and monitoring of marine pests in New Zealand. Aquatic Invasions 2012 Vol. 7 No. 1 pp. 125-128

Customer Comments

 "The PowerMax soil kits were really nice - we used them with a custom-made digestion buffer devised for ancient sediments. The post-extraction cleanup kit was also great - there is still some inhibitor carried over in most of the samples we have, but the kit nicely removes that. The PCRs worked generally fine afterwards. I hope we can start using the 96-well plates in 2014!" -  DR. MIKLÓS BÁLINT - Biodiversität und Klima Forschungszentrum (BiK-F)

Kit Components