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PowerMag® Soil DNA Isolation Kit

Overview

Automated isolation of DNA from soil, stool and environmental samples. Optimized for use with automated processing and liquid handling systems.
Order
Description Catalog # Price
4 x 96 preps 27100-4-EP $1,874.00    
4 x 96 preps (For KingFisher) 27000-4-KF $1,874.00    

Features

  • Optimized for tough samples – Automated isolation of high quality DNA from soil, stool and environmental samples
  • ClearMag® Technology – Novel magnetic particle technology captures DNA without binding organic inhibitors, facilitating isolation of pure DNA
  • Hands-free purification - Optimized for use with automated processing and liquid handling systems*
  • Inhibitor Removal Technology® - Removes PCR-inhibiting compounds, including humic substances, phenolics and polysaccharides

Description

The PowerMag® Soil DNA Isolation Kit is designed for automated isolation of microbial DNA from all soil types and other environmental samples, as well as stool, compost, sediment and manure. The protocol is designed for isolation of DNA from up to 0.25 g of sample and includes patented Inhibitor Removal Technology® to remove humic substances and other PCR inhibitors. Novel magnetic particle technology enables purification of DNA without the typical surface binding to the beads, eliminating the adsorption of organic inhibitors that is typical of other magnetic bead technologies, and facilitating isolation of pure DNA. In addition, neither chaotropic salts nor ethanol are used in the binding and washing steps, removing a second source of contamination that can inhibit enzymatic reactions.

*The PowerMag® Soil DNA Isolation Kit is designed for use with automated processing and liquid handling systems, such as the Thermo Scientific, Tecan, Hamilton, Beckman and Eppendorf systems. Protocols are available for the Thermo Scientific KingFisher and Eppendorf epMotion® platforms. For other automated liquid handling systems, please contact technical@mobio.com for assistance prior to purcasing the kit.

Download Application Note

Research and Development

PowerMag Soil Kit
Figure 1. High quality, pure DNA isolated from 0.25 g of organic garden soil using the PowerMag® Soil DNA Isolation Kit on the epMotion® 5075 TMX. High quality, high molecular weight DNA was observed in eight replicate samples examined on a 1% agarose gel. No differences in yield or quality were observed between the replicate samples. qPCR was performed using primers for Bacillus on 1 µl of DNA. Undiluted DNA and samples diluted 1:10 fell within the standard curve (grey lines), and the difference between the diluted and undiluted samples was approximately 3 cycles, indicating the DNA was free of inhibitors.
PowerMag Soil Kit
Figure 1. High quality, pure DNA isolated from a panel of six soil samples (0.25 g) using the PowerMag® Soil DNA Isolation Kit on the KingFisher® Duo. High quality, high molecular weight DNA was observed in each soil sample with yields varying based on microbial load. No differences in yield or quality were observed between the replicate samples. qPCR was performed on 1 µl of DNA from three soil types known to be high in PCR inhibitors, using primers for Bacillus. Undiluted DNA and samples diluted 1:10 fell within the standard curve (grey lines), and the difference between the diluted and undiluted samples was approximately 3 cycles, indicating the DNA was free of inhibitors.
PowerMag Soil Kit
Figure 2. Inhibitor Removal Technology® (IRT) is essential for removal of PCR inhibitors. DNA was isolated from organic garden soil samples using the PowerMag® Soil DNA Isolation Kit with and without the IRT step and purified using the KingFisher® Duo. qPCR was performed as described in Figure1. Samples processed without IRT failed to amplify, while samples with IRT were successfully amplified and fell within the standard curve (blue lines).
PowerMag® Soil DNA Isolation Kit
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Specifications

Format ClearMag® Technology
Method Bead Beating
Starting Amount 0.25 g
Throughput 96 well plate
Equipment Required
  • Plate shaker for homogenization of samples in 96 well blocks (recommended: MO BIO Catalog # 11996)
  • Centrifuge capable of handling two 96 well blocks
  • Automated processing or liquid handling system
  • Magnetic Separator (PowerMag® Magnetic Separator, Catalog # 27400 recommended for use with automated liquid handling systems, not required for KingFisher systems)

Storage Conditions

  • RNase A should be stored at 4°C for up to 2 years.
  • The other kit reagents and components should be stored at room temperature (15-30°C) for up to 2 years.