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PowerLyzer® PowerSoil® DNA Isolation Kit

Overview

Isolate genomic microbial DNA from environmental samples by using bead tubes that are optimized for maximum lysis with the PowerLyzer® 24 homogenizer.
Order
Description Catalog # Price
50 Preps 12855-50 $257.00    
100 Preps 12855-100 $494.00    

Features

  • Optimized lysis for tough samples - Isolate pure DNA from all soil samples and difficult sample types by using bead based homogenizers including the PowerLyzer® 24
  • Inhibitor Removal Technology® - Eliminates 100% of humic substances and PCR inhibitors for DNA that is ready to use in downstream applications
  • Rapid protocol - Enables isolation of high quality, pure DNA from 250 mg samples in just 30 minutes

Description

The PowerLyzer® PowerSoil® DNA Isolation Kit is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology® to extract microbial DNA from all soil types and other environmental samples. The isolated DNA has the highest level of purity allowing for more successful PCR and qPCR amplification of organisms from the sample. This kit contains bead tubes that are optimized for bead based homogenizers including the PowerLyzer® 24. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes.

The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.

Do not let the name fool you. Our customers use this kit successfully on diverse sample types such as food (cheese, milk, meat, fruit), water, insects and fecal samples in a variety of applications including biodefense, agriculture and zoology. These soil kits are used by the USEPA, USDA, FBI and CDC for soil analysis.

 

Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System! 

Sample Kit

Click to try a sample.

PowerLyzer® PowerSoil® DNA Isolation Kit
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MSDS

12855

Protocol

12855

Specifications

View the full PowerLyzer® PowerSoil® 
DNA Application Note

PowerLyzer PowerSoil DNA Application Note

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 20 µg per filter
Sample Size 250 mg
Throughput 1 - 24 samples
Time 30 minutes
Equipment Required Bead based homogenizer, like PowerLyzer 24

Storage Conditions

Store at room temperature for up to 2 years.

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