PowerLyzer™ PowerSoil® DNA Isolation Kit

Overview

For high clay soils, sediments and low biomass samples, or when using a high powered bead beater such as the PowerLyzer™ 24 homogenizer,  use the PowerLyzer™ PowerSoil^® DNA Isolation Kit.  This kit utilizes 0.1 mm glass bead tubes for enhanced homogenization of difficult soils, resulting in improved isolation of microbial DNA.  The PowerSoil^® and PowerLyzer™  PowerSoil^® DNA Isolation kits contain identical solutions and spin filters.

Description	Catalog #	Price
50 Preps	12855-50	$249.00
100 Preps	12855-100	$479.00

Related Products

PowerLyzer™ 24 Bench Top Bead-Based Homogenizer

Features

Next generation method improves PCR inhibitor removal efficiency

• Optimized for use with bead based homogenizers including the PowerLyzer^™ 2
•  Processes all soil types, including difficult environmental samples
• Patented technology removes 100% of humic substances and PCR inhibitors
•  Rapid protocol enables purification of DNA from 250 mg soil in 30 minutes
• Applications include biodefense, soil-borne pathogen detection, agriculture and zoology research

Description

The PowerLyzer^™ PowerSoil^® DNA Isolation Kit is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology^® to extract microbial DNA from all soil types and other environmental samples. The isolated DNA has the highest level of purity allowing for more successful PCR and QPCR amplification of organisms from the sample. This kit contains bead tubes that are optimized for bead based homogenizers including the PowerLyzer^™ 24. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes.

The PowerSoil^® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil^® DNA Isolation Kit and the UltraClean^® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.

Do not let the name fool you. Our customers use this kit successfully on diverse sample types such as food (cheese, milk, meat, fruit), water, insects and fecal samples in a variety of applications including biodefense, agriculture and zoology. These soil kits are used by the USEPA, USDA, FBI and CDC for soil analysis.

 

 

 NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System! 

Sample Kit

Click to try a sample.

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MSDS

12855

Protocol

12855

Specifications

View the full PowerLyzer™ PowerSoil^® 
DNA Application Note

Format	Silica Spin Filter Tubes
Method	Bead Beating
Binding Capacity	Up to 20 µg per filter
Sample Size	250 mg
Throughput	1 - 24 samples
Time	30 minutes
Equipment Required	Bead based homogenizer, like PowerLyzer™ 24

Storage Conditions

Store at room temperature for up to 2 years.

Publications

• Stewart, C. J., Nelson, A., Scribbins, D., Marrs, E. C. L., Lanyon, C., Perry, J. D.,Embleton N. D., Cummings S. P., Berrington, J. E. (2013). Bacterial and fungal viability in the preterm gut: NEC and sepsis. Archives of Disease in Childhood - Fetal and Neonatal Edition, doi: 10.1136/archdischild-2012-302119.

 

Kit Components

Component	12888-50
PowerLyzer™ Glass Bead Tubes, 0.1mm PowerLyzer™ PowerSoil® Bead Solution Solution C1 Solution C2 Solution C3 Solution C4 Solution C5 Solution C6 Spin Filters In Tubes Collection Tubes	50 42ml 1 x 3.3 ml 1 x 14 ml 1 x 11 ml 1 x 72 ml 1 x 27.5 ml 1 x 6 ml 50 200