Next generation method improves PCR inhibitor removal efficiency
The PowerLyzer™ PowerSoil® DNA Isolation Kit is our next generation soil DNA kit, which uses our novel, patented Inhibitor Removal Technology® to extract microbial DNA from all soil types and other environmental samples. The isolated DNA has the highest level of purity allowing for more successful PCR and QPCR amplification of organisms from the sample. This kit contains bead tubes that are optimized for bead based homogenizers including the PowerLyzer™ 24. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes.
The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that removes PCR inhibitors from even the most difficult soil types, promoting successful downstream PCR analysis. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.
Do not let the name fool you. Our customers use this kit successfully on diverse sample types such as food (cheese, milk, meat, fruit), water, insects and fecal samples in a variety of applications including biodefense, agriculture and zoology. These soil kits are used by the USEPA, USDA, FBI and CDC for soil analysis.
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DNA Application Note
|Format||Silica Spin Filter Tubes|
|Binding Capacity||Up to 20 µg per filter|
|Sample Size||250 mg|
|Throughput||1 - 24 samples|
|Equipment Required||Bead based homogenizer, like PowerLyzer™ 24|
|PowerLyzer™ Glass Bead Tubes, 0.1mm
PowerLyzer™ PowerSoil® Bead Solution
Spin Filters In Tubes
1 x 3.3 ml
1 x 14 ml
1 x 11 ml
1 x 72 ml
1 x 27.5 ml
1 x 6 ml