Isolation Kits by Sample

Soil
Microbial
Plants & Seeds
Tissue and cells/FFPE
Swab
Water
Biofilm
Blood
Fecal/Microbiome
Plasmids

Products by Category

Genomic DNA Isolation
RNA Isolation
Protein Extraction
High-throughput Purification
Dye Dots
DNA/RNA Clean-Up
Plasmid DNA Isolation
DNA-Free Reagents
Homogenization Equipment
Growth Media
Plastics & Spin Filters
Lab Supplies
Certified Water
Enzymes
Samples









PowerClean® Pro DNA Clean-Up Kit

Overview

New! Remove PCR inhibitors from purified DNA in just 7 minutes!

On sale now! Offer valid for web orders only through 9/30/14.
Order
Description Catalog # Price
50 preps 12997-50 $198.00 $158.00
You Save: $40.00 N/A
   

Features

  • Efficient secondary purification – Fast and easy 7 minute protocol to clean up your problematic DNA
  • Removes challenging impurities – Purifies nucleic acids containing humic substances, polyphenolics, polysaccharides and other PCR inhibitors
     
  • Successful amplification - High purity nucleic acids for use in PCR, qPCR and next generation sequencing

Description

New! Clean-up problematic DNA in half the time of the original PowerClean® DNA Clean-Up Kit

The PowerClean® Pro DNA Clean-Up Kit utilizes our patented Inhibitor Removal Technology® (IRT) to provide researchers with a novel and proprietary method for cleaning up previously isolated genomic DNA. This kit is a significantly streamlined improvement over the original PowerClean® DNA Clean-Up Kit, with a 7 minute protocol that includes fewer steps. Starting DNA may be amber to brown in appearance; an indicator of PCR inhibiting substances, particularly humics and polyphenols. Even samples that appear colorless may contain PCR inhibitors which can be cleaned up with this kit. The PowerClean® Pro DNA Clean-Up Kit will remove this brown color as well as any PCR inhibiting substances, such as humic acids, heme, polysaccharides, polyphenols, fulvic acids, lipids and dyes. The resulting high purity DNA is ready to use in the most demanding downstream applications, including PCR, qPCR and next generation sequencing.  This kit was validated with DNA isolated from a variety of problematic soils and also with DNA samples spiked with commercial humic acids.  However, it performs well on DNA isolated from virtually any sample source.

Archived or previously isolated DNA samples are purified when combined with our proprietary DNA Clean-Up reagents.  Inhibitors are selectively removed from the DNA solution. All DNA, including total genomic DNA, is captured on a silica membrane in a spin column format.  DNA is then washed and eluted from the membrane.  Percentage recovery varies depending on the level of inhibitors in the DNA that may be influencing the DNA yield measurement. Purified DNA is ready for PCR analysis and other downstream applications.  We recommend quantifying DNA via a PicoGreen assay or running DNA on an agarose gel to determine recovery yield. 

Sample Kit

Click to try a sample.

Research and Development

A.

[Starting]       [PowerClean® Pro]         [Zymo OneStep™]

B.

Sample 260/280 260/230
Starting Sample 1.36 0.84
PowerClean® Pro DNA 1.95 2.04
Zymo OneStep™ 1.55 1.18

 

Figure 1. Elimination of humic substances from DNA. Samples cleaned up with either the PowerClean® Pro DNA Clean-Up Kit or the Zymo Research OneStep™ PCR Inhibitor Removal Kit were examined on a 1.2% TAE gel (A). Following clean-up with the PowerClean® Pro DNA Kit, the samples were clear. However, samples processed with the OneStep™ Kit retained some brown color, indictaing that not all inhibitors were removed.(B). The concentration of the starting sample was observed to be artificially high, with low 260/280 and 260/230 ratios, indicating the presence of contaminants that absorb at A230, such as humic substances. Following clean-up with the PowerClean® Pro DNA Kit, the 260/280 and 260/230 ratios increased to levels consistent with pure DNA and the concentration of the DNA decreased to an average of 76.85 ng/μl, a value that was confirmed by quantitation using a Qubit PicoGreen Assay (data not shown). For samples cleaned-up with the OneStep™ Kit, the 260/280 and 260/230 ratios remained low, indicating that inhibitors were still present. Starting samples were brown due to the presence of humic substances 

 

Figure 2. Successful qPCR amplification. DNA described in Fig. 1 was examined via qPCR with a Bacillus 16s assay (1 µl of undiluted DNA cleaned up using two different methods, or 1:10 dilution). Samples cleaned up with the PowerClean® Pro DNA Kit were free of inhibitors, as indicated by successful amplification and a difference of approximately 3 cycles between the undiluted DNA and the 1:10 dilution. Undiluted samples cleaned up with the Zymo OneStep™ Kit failed to amplify, and 1:10 dilutions amplified with a higher Cq value than the PowerClean® Pro 1:10 dilution, indicating that PCR inhibitors remained in the samples.

PowerClean® Pro DNA Clean-Up Kit
Click over image to enlarge

MSDS

12997

Protocol

12997

Specifications

Format Silica Spin Filter Tubes
Method Secondary DNA Clean-up
Binding Capacity Up to 20µg per filter
Sample Size Up to 100µl of purified DNA
Through-put 1-24 Samples
Time 7 minutes
Equipment Required Vortex and Microcentrifuge

 Comparison with PowerClean® DNA Clean-Up Kit

  PowerClean® DNA Clean-Up Kit New PowerClean® Pro DNA Clean-Up Kit
Protocol Time 14 minutes       7 minutes

 Comparison with other Clean-Up Kits 

  PowerClean® Pro DNA

 PCR  Clean-Up

 GelSpin Ultra-Clean® 15
Silica Column X X X  

Silica Binding Particles

      X
Size Range 10bp - 50kb  60bp - 50kb  100bp - 50kb

 60bp - 50kb

Gel Extraction      X  X
Solution or Reaction Clean-Up  X  X  X  X
Capacity  20 µg  20 µg  Scalable  20 µg
Inhibitors Removed  Environmental, salts, proteins  Salts, proteins  Salts, Proteins  Salts, Proteins

Storage Conditions

Store at room temperature for up to 2 years.