PowerClean® DNA Clean-Up Kit

Overview

Related Products

Features

• Efficient secondary purification - cleans up your problematic archived DNA for successful PCR and QPCR amplification

• Removes challenging impurities - purifies DNA containing high amounts of humic substances, polyphenolics, polysaccharides and other PCR inhibitors

• Purifies concentrated DNA and removes concentrated contaminants

Description

The PowerClean^® DNA Clean-Up Kit provides a quick, easy and reliable secondary clean-up method to purify previously isolated genomic DNA from any source for all downstream applications. Problematic isolated DNA from soil may be amber to brown in appearance, which is an indicator of PCR inhibiting substances, like humics and polyphenols. Even colorless samples may contain PCR inhibitors which can be cleaned up with this kit. A high level of purity is achieved which allows for more successful PCR amplification from a variety of problematic samples.

This kit provides researchers with a novel method for cleaning up genomic DNA previously isolated from soil samples as well as archived DNA samples from any source. DNA extracted from many sample types including soil samples poses several challenges for downstream applications such as PCR, restriction digestion, cloning etc. The main limitation comes from humic acids and related compounds like polysaccharides, polyphenols, and heme which tend to co-purify along with nucleic acids. Phenol: chloroform extraction is not effective in purifying such contaminated nucleic acids while other methods are cumbersome and time consuming.

Sample Kit

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MSDS

12877

Protocol

12877

Specifications

Storage Conditions

Publications

• Mueller-Spitz, S.R., Goetz, G.W., and McLellan S. Temporal and Spatial Varibility in Nearshore Bacterioplankton Communities of Lake Michigan. FEMS Microbiology Ecology. 67(3):511-522 (2009).
• Nieto, M.P., and Carbone, S.S. Characterization of Juvenile Maritime Pine (Pinus pinaster Ait.) Ectomycorrhizal Fungal Community Using morphotyping, Direct Sequencing and Fruitbodies Sampling. Mycorrhiza. 19(2): 91-98 (2009).
• Elshahed, M.S., Youssef, N.H., Spain, A.M., Sheik, C., Najar, F.Z., Sukharnikov, L.O., Roe, B.A., Davis, J.P., Schloss, P.D., Bailey, V.L., Krumholz, L.R. Novelty and Uniqueness Patterns Of Rare Members Of The Soil Biosphere. Applied and Environmental Microbiology. (2008).

Kit Components