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UltraClean® Soil DNA Sample Kit
Overview
For isolation of genomic DNA from soil samples. PCR ready DNA in 30 minutes.*Limit 1 per order
Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 2 preps | 12800-S | $0.00 |
|
Features
- Established method selectively removes most PCR inhibitors
- Rapidly purify DNA from up to one gram of soil in 30 minutes
- Yields high quality PCR ready DNA up to 50 kb plus
- Includes a proprietary Inhibitor Removal Solution to eliminate humic acid inhibitors of PCR
- Efficiently lyses spores and fungi; also compatible with fecal samples
Description
The UltraClean® Soil DNA Kit has become the method of choice among researchers around the world studying microbial organisms in soil. This kit will isolate cellular, PCR quality DNA from soil and ensures removal of humic acid inhibitors.
Soil samples are added to a bead beating tube containing beads, lysis solution, bead solution and Inhibitor Removal Solution. The principal is to lyse the microorganisms in the soil by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action on a vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free Tris buffer.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per filter |
| Throughput | 1 - 24 samples |
| Time | 30 minutes |
with these Soil Types |
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Storage Conditions
Store at room temperature for up to 2 years.Kit Components
| Component | 12800-S |
|---|---|
| Bead Tubes Solution S1 IRS Solution Solution S2 Solution S3 Solution S4 Solution S5 Spin Filters In Coll. Tubes Collection Tubes |
2 1 x 140 µl 1 x 440 µl 1 x 550 µl 1 x 2.9 ml 1 x 700 µl 1 x 110 µl 2 6 |
