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UltraClean® Microbial RNA Sample Kit

Overview

For isolation of total RNA from microorganisms.
*Limit 1 per order
Order
Description Catalog # Price
2 preps 15800-S $0.00    

Features

  •  Isolates total RNA from microbial cells in 35 minutes
  • Yields high quality RNA ready for qPCR
  • Complete kit includes all required RNase-free tubes and ready to use reagents
  • Ideal for Gram positive and Gram negative bacteria, yeast, fungi and bacterial spores

Description

The UltraClean® Microbial RNA Isolation Kit is designed to yield up to 60 µg of high-quality RNA from pure cultures of microorganisms in 35 minutes. Based on a bead beating technology, cells are lysed, RNA is captured on a silica spin filter, washed and eluted in a certified RNase-free elution buffer, ready for reverse transcription and RT-PCR. This kit includes all required RNase-free tubes and reagents ready-to-use.

Requires our Vortex Adapter for maximum efficiency. This kit does not require organic solvents and is considered safer than TRIZOL®- based protocols.

UltraClean® Microbial RNA Sample Kit
Click over image to enlarge

MSDS

15800

Protocol

15800-S

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 60 µg per filter
Throughput 1 - 24 samples
Time 35 minutes

 

Microorganisms tested
with UltraClean Kit
  • B. subtilis
  • B. pumulis
  • E. coli
  • E. faecalis
  • P. aeruginosa
  • M. luteus
  • S. aureus
  • L. casei
  • A. niger
  • C. albicans
  • S. cerevisiae

Storage Conditions

Store Solution MR2 at 4°C. Store other components at room temperature for up to 2 years.

Kit Components

Component 15800-S
Bead Tubes
Solution MR1
Solution MR2
Solution MR3
Solution MR4
Solution MR5
RNase free Water
Spin Filters In Tubes
Collection Tubes
2
1 x 650 μl
1 x 40 μl
1 x 1.1 ml
1 x 600 μl
1 x 1 ml
1 x 100 μl
2
8