KAPA SYBR® FAST Universal 2x qPCR Master Mix Sample

Overview

Features

- KAPA SYBR® FAST qPCR Kits are ideally suited for:

- Gene expression analysis

- Low copy gene detection

- Microarray validation

- Gene knockdown validation

- High-throughput qPCR

- ChIP

- Next-generation DNA sequencing library quantification

Description

KAPA SYBR® FAST qPCR Kits are designed for the highest performance in real-time PCR.

The kits contain a novel DNA polymerase engineered through a process of molecular evolution - the result is a unique enzyme, specifically evolved for qPCR using SYBR® Green I dye chemistry.

The KAPA SYBR® DNA Polymerase was engineered to perform optimally in stringent real-time qPCR reaction conditions, exhibiting dramatic improvements to signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity, and sensitivity.

The KAPA SYBR® DNA Polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including both GC-rich and AT-rich templates.

SYBR® FAST qPCR Kits are supplied as a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The KAPA SYBR® FAST qPCR Kit is supplied as a 2x Master Mix with integrated antibody-mediated hot start, SYBR® Green I fluorescent dye, MgCl2, dNTPs, and stabilizers. 

KAPA SYBR® FAST qPCR Kits are intended for use on all real-time instruments and protocols.

* These enzymes are for research only.

Specifications

Cat # 51230-S: For 30 reactions. Supplied with separate 50X ROX High and 50X ROX Low (200 µl each).

* PLEASE NOTE FOR ALL PCR ENZYME SHIPPING, THERE WILL BE A $100.00 SHIPPING FEE FOR OVERNIGHT SERVICE AND PACKAGING MATERIALS.  OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY AND OUR REGULAR SHIPPING CHARGES WILL APPLY.

Storage Conditions

Store enzyme at -20°C for up to 1 year.