In less than 2.5 hours, the RNA PowerSoil® Total RNA Isolation Kit utilizes our novel and proprietary Inhibitor Removal Technology® (IRT) for isolating total RNA from up to 2 grams of soil. RT-PCR and PCR inhibiting substances, including humic substances and the brown color often associated with reverse transcription and PCR inhibition are completely removed. The kit is intended for use with all common soil types, but in particular, with environmental samples that typically contain high humic substance content, including compost, sediment and manure. RT-PCR was used to analyze undiluted total RNA purified using the RNA PowerSoil® Total RNA Isolation Kit. Primers were specific for Bacillus and Streptomyces spp. (see A, B C).
The kit offers a simple and easy-to-follow procedure. Environmental samples are added to a bead beating tube with a kit-supplied buffer for rapid and thorough homogenization. Total RNA is captured on a proprietary matrix in a column format, washed and eluted. RNA is RT-PCR and PCR ready without further handling or purification.
IMPORTANT NOTE: This kit requires the addition of phenol:chloroform (see protocol)