Genomic DNA Isolation
RNA Isolation
Protein Extraction
High-throughput Purification
Dye Dots
DNA Clean-Up
Plasmid DNA Isolation
DNA-Free Reagents
Homogenization Equipment
Growth Media
Plastics & Spin Filters
Lab Supplies
Certified Water
Enzymes
SamplesThe PowerSoil® DNA Isolation Kit uses patented Inhibitor Removal Technology® to isolate microbial genomic DNA from all soil types and environmental samples, as well as fecal, stool and biosolid samples. The isolated DNA has the highest level of purity allowing for more successful PCR and QPCR amplification of organisms from the sample. The kit is ideal for processing all environmental samples including difficult types containing a high humic acid content such as compost, sediment, and manure. PCR analysis has been performed to detect a variety of organisms including both Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces), and nematodes. The PowerSoil® DNA Isolation Kit distinguishes itself from other kits with a patented humic substance/brown color removal procedure that eliiminates PCR inhibitors from even the most difficult soil types, enabling samples to be used for the most demanding downstream applications, including PCR, QPCR and next generation sequencing. When choosing between the PowerSoil® DNA Isolation Kit and the UltraClean® Soil DNA Isolation Kit, use this kit for samples that are very high in humic content.
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per filter |
| Sample Size | 250 mg |
| Throughput | 1 - 24 samples |
| Time | 30 minutes |
| Equipment Required | Vortex and Vortex Adapter |