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PowerPlant® Pro DNA Isolation Kit Sample

Overview

For fast and easy isolation of inhibitor-free genomic DNA from even the toughest plant and seed samples, including those high in polyphenols and polysaccharides.
*Limit 1 per order.
Order
Description Catalog # Price
2 preps 13400-S $0.00    

Features

  • Optimized for tough samples - Isolate DNA from the most difficult plant types, including strawberry leaf, cotton leaf, cotton seeds and pine needles
  • Inhibitor Removal Technology® - Removes 100% of polyphenolics, polysaccharides and other PCR inhibitors
  • Phenolic Separation Solution - Breaks the bond between DNA and phenolics, increasing DNA yield in high-phenolic samples
  • Rapid protocol - Isolate DNA in 30 minutes without the use of harsh chemicals

Description

Isolate pure, high quality DNA from the toughest plant samples

The PowerPlant® Pro DNA Isolation Kit is designed for fast and easy purification of genomic DNA from plant cells, tissues and seeds. The optimized bead beating technology replaces cumbersome DNA isolation procedures such as CTAB, phenol, or chloroform extraction for recovery of high quality DNA from the toughest sample types, including strawberry leaf, cotton leaf, cotton seeds, and pine needles.

Patented Inhibitor Removal Technology® (IRT) is used for removal of PCR inhibitors from plant extracts during the isolation process, resulting in pure DNA that is ready to use in downstream applications including PCR, QPCR and sequencing. In addition, a unique Phenolic Separation Solution (PSS) is included as an optional step for samples high in polyphenolic compounds, such as pine and grape leaf. PSS breaks the bond between DNA and phenolics, preventing loss of DNA during the IRT step and increasing DNA yield.

Compatible with the PowerLyzer® 24

The PowerPlant® Pro DNA Isolation Kit may be used with a vortex (for soft leaf tissue) or high velocity bead beater, such as the PowerLyzer® 24 homogenizer. The PowerLyzer® 24 is suitable for rapid homogenization of plant materials including stems, roots, seeds or difficult leaf tissue.

PowerPlant® Pro DNA Isolation Kit Sample
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MSDS

 13400

Protocol

13400-S

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Starting Amount Up to 50 mg
Binding Capacity Up to 40 µg per filter
Throughput 1 - 24 samples
Time 30 minutes
Equipment Required

Vortex & Adapter* or PowerLyzer® 24

Centrifuge

*For soft leaf tissue

Storage Conditions

RNase A should be stored at 4°C for up to 2 years

The other kit reagents and components should be stored at room temperature (15-30°C) for up to 2 years