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- Purifies DNA from 10 g soil in 30 minutes - means NO time spent on multiple small sample processing
- Increases detection sensitivity on samples with a low microbial load
- Provides high quality DNA that is free of PCR inhibitors including humic substances
- Purified samples are suitable for PCR and QPCR
The PowerMax® Soil DNA Isolation Kit is our next-generation kit designed for processing large quantities of any soil or environmental sample with high or low microbial load. Based on reagents from the PowerSoil® DNA Isolation Kit, the PowerMax® Soil DNA Isolation Kit uses our novel, patented Inhibitor Removal Technology® to remove PCR inhibiting compounds, including humic substances, and the brown color often associated with soil DNA. The kit is ideal for environmental samples that contain high humic substance content, including compost, sediment and manure. PCR analysis has been used to amplify DNA purified from microorganisms including Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces).
The PowerMax® Soil DNA Isolation Kit increases your ability to study microbial diversity in even the most difficult sample types, such as those which contain high humic levels or low microbial load. Our 50 ml Vortex Adapter for cell lysis and a centrifuge capable of spinning 50 ml tubes are required for maximum performance.
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Sample Size | Up to 10 grams |
| Binding Capacity | Up to 1 mg per filter |
| Throughput | 1 - 24 samples |
| Time | 30 minutes |
| Equipment Required | Vortex and Centrifuge |
| Soil Samples Tested | |
|---|---|
| Aquifer Sediment | Manure |
| Clay | Marine Sediment |
| Fecal Intestinal Tract | Oil-saturated Soil |
| Forest | Potting Soil |
| Fungal Mat | Sand |
| Loam | Sewer Sludge |