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PowerMax® Soil DNA Isolation Kit Sample

Overview

For efficiently isolating microbial DNA of high quality and purify from large quantities of soil - great for samples with low microbial load.
Order
Description Catalog # Price
2 preps 12988-S $49.00    

Features

  • Optimized detection from tough samples - Increases detection sensitivity from samples with low microbial load
  • Inhibitor Removal Technology® - Eliminates 100% of humic substances and other PCR inhibitors, for DNA that is ready to use in PCR, qPCR and next generation sequencing
  • Rapid protocol - Enables isolation of high quality, pure DNA from 10 g soil in just 30 minutes

Description

The PowerMax® Soil DNA Isolation Kit is our next-generation kit designed for processing large quantities of any soil or environmental sample with high or low microbial load. Based on reagents from the PowerSoil® DNA Isolation Kit, the PowerMax® Soil DNA Isolation Kit uses our novel, patented Inhibitor Removal Technology® to remove PCR inhibiting compounds, including humic substances, and the brown color often associated with soil DNA. The kit is ideal for environmental samples that contain high humic substance content, including compost, sediment and manure. PCR analysis has been used to amplify DNA purified from microorganisms including Gram-positive and Gram-negative bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae, and actinomycetes (e.g. Streptomyces).

The PowerMax® Soil DNA Isolation Kit increases your ability to study microbial diversity in even the most difficult sample types, such as those which contain high humic levels or low microbial load. Our 50 ml Vortex Adapter for cell lysis and a centrifuge capable of spinning 50 ml tubes are required for maximum performance.

PowerMax® Soil DNA Isolation Kit Sample
Click over image to enlarge

MSDS

12988

Protocol

12988-S

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Sample Size Up to 10 grams
Binding Capacity Up to 1 mg per filter
Throughput 1 - 24 samples
Time 30 minutes
Equipment Required Vortex and Centrifuge

Soil Samples Tested  
Aquifer Sediment Manure
Clay Marine Sediment
Fecal Intestinal Tract Oil-saturated Soil
Forest Potting Soil
Fungal Mat Sand
Loam Sewer Sludge