The PowerLyzer™ UltraClean® Tissue RNA Isolation Kit is designed to provide efficient purification of total RNA from a variety of mammalian tissues or cultured cells.
Prepared fresh or frozen tissues are homogenized in 2.8mm ceramic bead tubes using a bead based homogenizer such as the PowerLyzer™ 24, in the presence of lysis solutions. The RNA is then bound to a silica spin filter, washed and eluted into certified RNase-free water (provided). Kit includes all RNase-free reagents ready to use and all required spin filters and tubes. RNA is of high quality and is ready for any downstream applications such as qPCR.
Requires our PowerLyzer™ 24 for maximum efficiency.
|Format||Silica Spin Filter Tubes|
|Method||Bead Based Homogenization|
|Starting Amount||10 to 30 mg|
|Binding Capacity||Up to 60 µg per filter|
|Throughput||1 - 24 samples|