PowerLyzer™ UltraClean® Plant RNA Sample Kit

Overview

Features

- Rapid isolation of total RNA from plant tissues in only 35 minutes

- Contains 2.8mm ceramic bead tubes optimized for use with bead based homogenizers such as the PowerLyzer^™ 24

- Yields up to 60 μg high quality RNA for RT and RT-PCR

- Safe and easy protocol uses NO phenol/chloroform extraction, CsCl gradients or ethanol precipitation

- Includes all ready-to-use RNase-free reagents and all required spin filters and tubes

Description

The PowerLyzer^™UltraClean^® Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials and contains 2.8mm ceramic bead tubes for homogenization with bead-based homogenizers such as the PowerLyzer^™ 24. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.

Fresh or frozen plant tissues are homogenized using a bead based homogenizer like the PowerLyzer^™ 24. After cell lysis, the released RNA is captured on a silica membrane spin filter. The RNA bound to the filter is washed to remove all contaminants. Finally, the RNA is recovered in certified RNase-free water (provided). The eluted RNA is very pure and ready for sensitive applications such as reverse-transcription PCR.

MSDS

13355

Protocol

13355-S

Specifications

Storage Conditions

Store at room temperature for up to 2 years