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KAPA2G FAST HotStart ReadyMix Sample

Overview

2G Fast is well suited for both routine and high-throughput PCR, achieving higher yields and improved sensitivity than competitor enzymes across a wide range of amplicon types.
*Available for purchase to US direct customers only
Order
Description Catalog # Price
20 x 25 µl reactions 51270-S $35.00    

Features

- Extension times as low as 1 sec per kilobase

- Outstanding performance when compared to conventional hot start Taq polymerase

- 20 - 70% reductions in total PCR time

- No requirement for specialist PCR consumables or instrumentation.

Description

KAPA2G Fast DNA Polymerase, a second-generation enzyme derived through a process of molecular evolution.

KAPA2G Fast DNA Polymerase was specifically engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step.

This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency.

KAPA2G Fast HotStart is particularly well suited for high throughput Fast PCR, offering total reaction times 20 - 70% shorter than conventional PCR assays performed with wild-type Taq polymerase or hot start formulations thereof. In addition, KAPA2G Fast HotStart has been shown to achieve higher yields and sensitivity than competitor enzymes across a wide range of amplicon types. No specialized PCR consumables or thermocyclers are required.

 * These enzymes are for research only.

 

KAPA2G FAST HotStart ReadyMix Sample
Click over image to enlarge

Specifications

Cat # 51270-S: 20 x 25µl rxns. Supplied with KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.

Disclaimer: MO BIO Laboratories, Inc. is an authorized distributor of Kapa Biosystems.

* PLEASE NOTE FOR ALL PCR ENZYME SHIPPING, THERE WILL BE A $100.00 SHIPPING FEE FOR OVERNIGHT SERVICE AND PACKAGING MATERIALS.  OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY AND OUR REGULAR SHIPPING CHARGES WILL APPLY.

Storage Conditions

Store enzyme at -20°C for up to 1 year.

Customer Comments

"This shaved 52.5 minutes off of our normal thermal protocol, plus additional time for the heating/cooling. Thanks for giving us a trial of this product! I'm sure that I'll be using it in the future.