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UltraClean® Tissue & Cells DNA Sample Kit

Overview

For isolating genomic DNA from animal tissues, rodent tails and cultured cells without using organic solvents.
*Limit 1 per order
Order
Description Catalog # Price
2 preps 12334-S $0.00    

Features

  • Rapidly isolate high quality and high molecular weight DNA from animal tissues or cultured cells in less than 20 minutes without the use of hasardous organic solvents
  • DNA is ready for PCR, restriction digests, and all downstream applications
  • Novel bead beating method for hands-off homogenization
  • Lyophilized Proteinase K has been included in the kit for processing tough tissue samples

Description

The UltraClean® Tissue & Cells DNA Isolation Kit is ideal for isolating genomic DNA from cultured cells or animal tissues, including rodent tails. Isolated DNA is high quality and ready for PCR, restriction digests, and other downstream applications. The UltraClean® Tissue & Cells DNA Isolation Kit is safe and user-friendly because it avoids the use of organic solvents like phenol and chloroform.

The UltraClean® Tissue & Cells DNA Isolation Kit is designed for isolating DNA from 1-25 mg tissue samples or up to 5 x 106 cells.

Fresh or frozen tissue samples are homogenized using bead beating technology to lyse the cells. Lysates are loaded onto a silica spin filter. DNA binds to the silica spin filter while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed by a wash step. Pure DNA is then eluted into certified, DNA-free Tris buffer.This kit requires a microcentrifuge, vortex and our Vortex Adapter.

This kit contains an optional step for tough tissue samples such as bone, hair and mouse tails. Lyophilized Proteinase K has been included in this kit for processing these tissue samples.
 

UltraClean® Tissue & Cells DNA Sample Kit
Click over image to enlarge

MSDS

12334

Protocol

12334-S

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Starting Amount 25 mg
Binding Capacity Up to 40 µg per filter
Throughput 1 - 24 samples
Time <20 minutes

 

Animal Tissues Tested
with UltraClean Tissue Kits
  • Brain
  • Lung
  • Mouse Tails
  • Liver
  • Pancreas
  • Heart
  • Spleen
  • Gallbladder
  • Bone Marrow

 

Storage Conditions

Store at room temperature for up to 2 years.

Kit Components

Component 12334-S
Dry Bead Tubes 2
Solution TD1 2.8 ml
Solution TD2 1 ml
Solution TD3 220 µl
Proteinase K Solution 44 µl
Spin Filters 2
2 ml Collection Tubes 4