The UltraClean® Endotoxin-Free Mini Plasmid Prep Kit* integrates an efficient endotoxin removal step into the plasmid purification procedure to make it the fastest endotoxin-free mini prep method available. Purified, endotoxin-free DNA is suitable for transfection and injection.
Large numbers of clones that require endotoxin-free DNA can be rapidly purified using this unique miniprep kit. For larger isolation needs, use our midi or maxi prep kits.
No additional affinity columns to remove lipopolysaccharides are required. Cells are lysed with an improved set of alkaline lysis reagents. Endotoxins are selectively precipitated in a specialized buffer. The plasmid DNA is bound to a silica spin filter, washed once and released into endotoxin-free water (provided). The plasmid DNA is concentrated enough to be used directly in transfection experiments.
Endotoxins, also known as lipopolysaccharides, are cell membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxins represent a non-controllable variable in transfection experiment setup, making results difficult to compare and interpret.
* DNA isolated with this kit should be used for research purposes only.
|Format||2ml Silica Spin Filter Tubes|
|Method||Alkaline Lysis/Endo removal|
|Binding Capacity||Up to 20 µg per filter|
|Throughput||1 - 24 samples|
|Endotoxin Level||<0.1 EU/µg|
Spin Filters In Tubes
|1 x 220 µl
1 x 440 µl
1 x 660 µl
1 x 270 µl
1 x 1.5 ml
1 x 660 µl
1 x 110 µl