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UltraClean® Endotoxin-Free Mini Plasmid Sample Kit

Overview

For isolation of up to 20 µg of endotoxin-free UltraClean® plasmid DNA.
*Limit 1 per order
Order
Description Catalog # Price
2 preps 12311-S $0.00    

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Features

  • Results in high quality plasmid DNA containing less then 0.1 EU/µg in less time
  • Produces DNA suitable for transfection and injection
  • Utilizes a simple silica spin filter for DNA yields equal to anion exchange columns
  • Rapid protocol with no additional affinity columns required to remove lipopolysaccharides

Description

The UltraClean® Endotoxin-Free Mini Plasmid Prep Kit* integrates an efficient endotoxin removal step into the plasmid purification procedure to make it the fastest endotoxin-free mini prep method available. Purified, endotoxin-free DNA is suitable for transfection and injection.

Large numbers of clones that require endotoxin-free DNA can be rapidly purified using this unique miniprep kit. For larger isolation needs, use our midi or maxi prep kits.

No additional affinity columns to remove lipopolysaccharides are required. Cells are lysed with an improved set of alkaline lysis reagents. Endotoxins are selectively precipitated in a specialized buffer. The plasmid DNA is bound to a silica spin filter, washed once and released into endotoxin-free water (provided). The plasmid DNA is concentrated enough to be used directly in transfection experiments.

Endotoxins, also known as lipopolysaccharides, are cell membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxins represent a non-controllable variable in transfection experiment setup, making results difficult to compare and interpret.


* DNA isolated with this kit should be used for research purposes only.

UltraClean® Endotoxin-Free Mini Plasmid Sample Kit
Click over image to enlarge

MSDS

 12311

Protocol

12311-S

Specifications

Format 2ml Silica Spin Filter Tubes
Method Alkaline Lysis/Endo removal
Binding Capacity Up to 20 µg per filter
Throughput 1 - 24 samples
Time 10 minutes
Endotoxin Level <0.1 EU/µg

Storage Conditions

Store at room temperature for up to 2 years.

Kit Components

Component 12311-S
Solution EF1
Solution EF2
Solution EF3
Solution EF4
Solution EF5
Solution EF6
Solution EF7
Spin Filters In Tubes
Collection Tubes
1 x 220 µl
1 x 440 µl
1 x 660 µl
1 x 270 µl
1 x 1.5 ml
1 x 660 µl
1 x 110 µl
2
12