Isolation Kits by Sample

Soil
Microbial
Plants & Seeds
Tissue and cells/FFPE
Swab
Water
Biofilm
Blood
Fecal/Microbiome
Plasmids

Products by Category

Genomic DNA Isolation
RNA Isolation
Protein Extraction
High-throughput Purification
DNA/RNA Clean-Up
Plasmid DNA Isolation
DNA-Free Reagents
Homogenization Equipment
Growth Media
Plastics & Spin Filters
Lab Supplies
Certified Water
Enzymes
Samples









RTS DNase™ Kit Sample

Overview

Room Temperature Stable - for the removal of genomic DNA contamination in RNA preparations.
*Limit 1 per order.
Order
Description Catalog # Price
4 preps 15200-S $0.00    

Related Products

RTS DNase™ Kit

Features

  • Room Temperature Stable DNase – Eliminates freeze-thaws to maintain enzyme activity at the highest level
  • Protects RNA – Certified RNase free, no heat, EDTA or harsh chemicals required
  • DNase Removal Resin – Completely removes DNase and divalent cations for optimal QPCR results
  • Fast – 30 minute protocol from start to finish

 

Description

RTS (Room Temperature Stable) DNase is a highly purified DNase I enzyme formulated in a unique stabilization solution that provides long term stability at room temperature. The RTS DNase™ Kit is used for the removal of genomic DNA contamination in RNA preparations. RTS DNase will remove up to 30 µg of DNA in 20 minutes using 10 units (1 µl) of enzyme. The enzyme is stable for up to 6 months at room temperature with no loss of activity and for 2 years at 4°C without loss of activity. The RTS DNase™ Kit also contains a novel and highly specific resin which is used to bind and remove the RTS DNase enzyme and divalent cations from the reaction, eliminating the need for heat or EDTA inactivation of the DNase. The RNA is ready to use immediately after resin treatment.  

Preserve enzyme activity

RTS DNase is the first DNase I enzyme which is stable at room temperature, so there is no need to aliquot and freeze stocks of the enzyme. Room temperature stability eliminates concern about freeze-thaw cycles that may decrease enzymatic activity. RTS DNase maintains the highest activity over the life of the kit, enabling consistent results with all RNA samples.

Protect your RNA

Isolating high quality RNA is a time-consuming and expensive process. Consequently, it is essential to prevent RNA degradation during DNase treatment. The RTS DNase™ kit protects valuable RNA samples during this process, as it contains only Certified RNase-free reagents, and does not require heat, EDTA or harsh chemicals.

Protocol Overview

The RTS DNase™ Kit protocol starts with dilution of RTS DNase and 10X RTS DNase buffer to a final concentration of 1X in the digestion reaction. The reaction is then incubated at 37°C for 20 minutes. Removal of the DNase and divalent cations is performed by adding RTS DNase Removal Resin and incubating for 10 minutes at room temperature. The resin is pelleted by centrifugation, and the RNA sample is transferred to a new tube, ready for use in RT-PCR and further analysis.

RTS DNase™ Kit Sample
Click over image to enlarge

MSDS

 15200

Protocol

 15200

Specifications

Unit Definition 10 units/µl, removes 30µg DNA
Time 30 minutes
Stability 6 months at room temperature
  2 years at 4ºC
Equipment Required Centrifuge
Equipment Recommended Vortex Genie® 2 Vortex
  Vortex adapter for 2 ml tubes

Storage Conditions

Store the RTS DNase™ Kit at 4ºC for up to 2 years, or at room tempearture for up to 6 months.