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PowerClean® Pro RNA Clean-Up Kit Sample

Overview

New! Remove inhibitors from purified RNA in just 7 minutes!
*Limit 1 per order
Order
Description Catalog # Price
2 preps 13997-S $0.00    

Features

  • Efficient secondary purification – Fast and easy 7 minute protocol to clean up your problematic RNA
  • Removes challenging impurities – Purifies RNA containing humic substances, polyphenolics, polysaccharides and other PCR inhibitors
     
  • Successful amplification - High purity RNA for use in PCR and RT-qPCR
  •  

Description

New! The only commercially available kit dedicated to secondary RNA purification.

The PowerClean® Pro RNA Clean-Up Kit utilizes our patented Inhibitor Removal Technology® (IRT) to provide researchers with a novel and proprietary method for cleaning up previously isolated RNA. Starting RNA may be amber to brown in appearance; an indicator of PCR inhibiting substances, particularly humics and polyphenols.  Even samples that appear colorless may contain PCR inhibitors which can be cleaned up with this kit. The PowerClean® Pro RNA Clean-Up Kit will remove this brown color as well as any PCR inhibiting substances, such as heme, polysaccharides, polyphenols fulvic acids and dyes.  A high purity of RNA is achieved allowing for more successful RT-PCR amplification of RNA derived from organisms in the original sample. This kit was validated with RNA isolated from a variety of problematic soils and also with RNA samples spiked with commercial humic acids.  However, it performs well on RNA isolated from virtually any sample source. 

Archived or previously isolated RNA samples are purified when combined with our proprietary RNA Clean-Up reagents. Inhibitors are selectively removed from the RNA solution. All RNA is captured on a silica membrane in a spin column format.  RNA is then washed and eluted from the membrane. Percentage recovery varies depending on the level of inhibitors that may be influencing the RNA yield measurement. Purified RNA is ready for RT-PCR analysis and other downstream applications.

Research and Development

A.

B.

Sample ng/µl 260/280 260/230
Starting Sample 292.85 1.88 0.56
1 126.60 2.09 2.35
2 130.36 2.08 2.36
3 129.14 2.11 2.37
4 133.63 2.09 2.35

Figure 1. Elimination of humic acids from RNA. The amount, quality and molecular weight of RNA visualized on the gel was similar for the starting sample and the samples following clean up (A). The starting sample contained humic acids, which absorb at A230. Following clean-up, the 260/280 and 260/230 ratios increased to levels consistent with pure RNA and the concentration of the RNA decreased to an average of 129.93 ng/μl, a value that was confirmed by quantitation using a Qubit PicoGreen Assay (data not shown).

Figure 2. Successful RT-qPCR. RNA described in Fig. 1 was examined via RT-qPCR with a Bacillus 16S assay (1l of undiluted RNA or a 1:10 dilution). Samples were free of inhibitors, as indicated by successful amplification of the undiluted RNA and a difference of approximately 3 cycles between the undiluted and the 1:10 dilution. Starting samples and 1:10 dilutions failed to amplify due to the presence of inhibitors.

PowerClean® Pro RNA Clean-Up Kit Sample
Click over image to enlarge

MSDS

13997

 

Protocol

13997

Specifications

Format Silica Spin Filter Tubes
Method Secondary RNA Clean-Up
Binding Capacity Up to 20µg per filter
Sample Size Up to 100µl of purified RNA
Through-put 1-24 Samples
Time 7 minutes
Equipment Required Vortex and Vortex Adapter, Microcentrifuge

Storage Conditions

Store at room temperature for up to 2 years.