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RNase Detection Kit
Overview
Highly sensitive detection of RNase contaminationOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 1 Test Kit (4 samples) | 12073-1 | $125.00 |
|
| 2 Test Kit (8 samples) | 12073-2 | $216.00 |
|
| 5 Test Kit (20 samples) | 12073-5 | $509.00 |
|
Features
* DUE TO THE TEMPERATURE SENSITIVE NATURE OF THIS PRODUCT, WE ARE UNABLE TO SHIP OUTSIDE THE US.
- Rapid detection protocol takes less than 1.5 hours.
- Test can detect as low as 10-9 Kunitz units/µl.
- User friendly protocol is as simple as performing an enzymatic digest and running an agarose gel.
- All kit components are certified RNase-Free.
- All tubes and reagents required are included in kit and ready to use including individually wrapped pipet tips.
Description
The RNase Detection Kit* sensitively detects RNase contamination present in sample solutions, to a level of 10-9 Kunitz units/µl. The procedure involves incubating control RNA with test samples, followed by agarose gel analysis using included control standards. The kit is certified RNase-free and is appropriate for screening reagent solutions for RNase activity prior to use with RNA samples. Shipped on dry ice. Must be kept frozen before use.
Up to four samples can be tested simultaneously with this kit. RNase activity can be quantified by comparison to the reference standards included. The kit is ideal for early detection of RNase activity in reagent solutions that will be used for sensitive RNA based experiments.
The procedure is to mix 1 µl of the test sample with an RNA standard provided. The sample is incubated at 37°C for 1 hour. After incubation, the sample is loaded onto a native agarose gel along with four control standards included with the kit. Results are interpreted from the gel analysis.
The procedure is to mix 1 µl of the test sample with an RNA standard provided. The sample is incubated at 37°C for 1 hour. After incubation, the sample is loaded onto a native agarose gel along with four control standards included with the kit. Results are interpreted from the gel analysis.
Research and Development
| 1 | 2 | 3 | 4 | 5 | 6 |
of RNA degradation as compared to complete
degradation by the positive control (lane 6).
- Lane 1: Test sample #1
Lane 2: Test sample #2
Lane 3: Test sample #3
Lane 4: Test sample #4
Lane 5: Negative control
Lane 6: Positive control
Click over image to enlarge
MSDS
Protocol
Specifications
Test Sensitivity
The RNase Detection Kit is capable of detecting as low as 10-9 Kunitz units/µl. For clarity, 1 unit of RNase is commonly defined as the amount of enzyme that produces an increase of 1.0 A260 unit at 37°C when RNA is hydrolyzed to acid soluble oligonucleotides.
One Kunitz unit is equal to 50 RNase units. The increase of 1.0 A260 unit represents the loss of
40 µg/ml (0.04 µg/µl) or 1/50 Kunitz unit.
* PLEASE NOTE FOR ALL DNASE & RNASE DETECTION KITS, THERE WILL BE A $75.00 HANDLING FEE IN ADDITION TO THE OVERNIGHT SHIPPING CHARGE. THIS WILL BE DETERMINED BASED ON YOUR DOMESTIC SHIPPING ADDRESS WITHIN THE CONTINENTAL UNITED STATES. DUE TO THE TEMPERATURE SENSITIVE NATURE OF THIS PRODUCT, IT HAS TO SHIP NEXT DAY AIR. OTHER MATERIALS IN YOUR ORDER WILL BE SHIPPED SEPARATELY AND OUR REGULAR SHIPPING CHARGES WILL APPLY.
Storage Conditions
Store kit at -20°C to -70°C for up to 6 months.
Long term storage is recommended at -70°C.
RNase Detection Kit is shipped on dry ice.
Kit Components
| Standard A | 4 x 10 µl | 8 x 10 µl | 20 x 10 µl |
| Standard B | 1 x 10 µl | 2 x 10 µl | 5 x 10 µl |
| Control C | 1 x 12 µl | 2 x 12 µl | 5 x 12 µl |
| Control D | 1 x 12 µl | 2 x 12 µl | 5 x 12 µl |
| Control E | 1 x 12 µl | 2 x 12 µl | 5 x 12 µl |
| Gel Dye | 1 x 20 µl | 2 x 20 µl | 5 x 20 µl |
| Pipet Tips | 20 | 40 | 100 |
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