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PowerPlant® RNA Isolation Kit with DNase

Overview

For fast and easy isolation of inhibitor-free total RNA from even the toughest plant and seed samples. Includes all reagents necessary to perform DNase treatment.

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Order
Description Catalog # Price
50 preps 13550-50 $343.00    

Features

  • Optimized for tough samples - Isolate RNA from the most difficult plant types, including strawberry leaf, cotton leaf, cotton seeds and pine needles
  • Inhibitor Removal Technology®  -  Removes 100% of polyphenolics, polysaccharides and other PCR inhibitors
  • Phenolic Separation Solution - Breaks the bond between RNA and phenolics, increasing RNA yield in high-phenolic samples
  • Rapid protocol - Isolate RNA in 30 minutes without the use of harsh chemicals
  • Complete Kit - Includes DNase I enzyme and reagents to perform DNase treatment 

Description

Isolate pure, high quality RNA from the toughest plant types

The PowerPlant® RNA Isolation Kit with DNase is designed for fast and easy purification of total RNA from plant cells, tissues and seeds. The bead beating technology used in this kit replaces potentially damaging RNA isolation procedures such as CTAB and TRIZOL extraction for recovery of high quality RNA from the toughest sample types, including strawberry leaf, cotton leaf, cotton seeds, and pine needles. Patented Inhibitor Removal Technology® (IRT) is used for removal of PCR inhibitors from plant extracts during the isolation process, resulting in pure RNA that is ready to use in all downstream applications. In addition, a unique Phenolic Separation Solution (PSS) is included as an optional step for samples high in polyphenolic compounds, such as pine and grape leaf. PSS breaks the bond between RNA and phenolics, preventing loss of RNA during the IRT step and increasing RNA yield.

You can also isolate miRNA using this kit. See modified protocol here

Supplied with DNase I enzyme for convenient, on-column removal of genomic DNA

The PowerPlant® RNA Isolation Kit with DNase contains all reagents needed to perform an on-column DNase treatment to remove contaminating genomic DNA. DNase treatment takes just 15 minutes, and eluted RNA is ready to use in any downstream application.

Compatible with the PowerLyzer® 24

The PowerPlant® RNA Isolation Kit with DNase may be used with a vortex (for soft leaf tissue) or high velocity bead beater, such as the PowerLyzer® 24 homogenizer. The PowerLyzer® 24 is suitable for rapid homogenization of plant materials including stems, roots, seeds or difficult leaf tissue.

 

Sample Kit

Click here to try a sample. Sample kit does not include reagents for DNase treatment.

Research and Development

Figure 1. The PowerPlant® RNA isolation kit provides higher yields of intact RNA from tough samples. Total RNA was isolated using the PowerPlant® RNA Isolation Kit and plant RNA isolation kits from two other suppliers. All isolations were performed according to the manufacturer’s protocol. RNA samples from cotton leaf (8 µl) and seed (2 µl) were examined on a 1% TAE agarose gel.

Figure 2. Successful amplification of RNA isolated using the PowerPlant® RNA Isolation Kit. RT-qPCR was performed using primers for RuBisCo on 1 ng of RNA isolated from cotton leaf (A) and seed (B). All samples fell within the standard curve (purple lines). 

PowerPlant® RNA Isolation Kit with DNase
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More images
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MSDS

13550

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Starting Amount Up to 50 mg
Binding Capacity Up to 40 µg per filter
Throughput 1 - 24 samples
Time 

30 minutes (RNA Isolation)

15 minutes (DNase treatment)

Equipment Required

Vortex & Adapter* or PowerLyzer® 24

Centrifuge

*For soft leaf tissue

 

Storage Conditions

Store lyophalized DNase I at 4°C and reconstituted DNase I at -20°C
Store all other kit components at room temperature (15-30°C)