PowerBiofilm™ RNA Isolation Kit

Overview

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Features

• Complete lysis: Pretreatment + mechanical lysis (bead beater or vortex) + chemical lysis

• Patented IRT inside: Allow inhibitor-free purified RNA for downstream applications

• Consistent high yields: more successful preps for better performance in downstream applications

•  Compatible with most biofilms: from dental plaques to microbial mats

Description

The PowerBiofilm™ RNA Isolation Kit from MO BIO Laboratories, Inc., has been specifically designed to overcome inefficient cell lysis. The key to the success of these kits is our new bead tube formulation that combines brand-new chemical and mechanical lysis.

Next, we’ve paired this with our Inhibitor Removal Technology® (IRT) to remove the concentrated inhibitors (such as humic acid, metals, salts and pesticides) typically present in biofilms.

For more information, visit www.mobio.com/biofilm.

*This product is for research use only

Sample Kit

Click to try a sample

Research and Development

Successful Isolation of RNA From Diverse Sample Types

*All yields represent combined results from multiple experiments and collaborators.

 
Successful RT-PCR

RNA was reverse transcribed using the QuantiTech Reverse Transcription Kit (Qiagen).Primers used were universal for the 16S rRNA gene (StreF/StreR) and generated a 1,243 bp product. The KAPA Fast HotStart kit was used for PCR. 

Sensitive Real-Time

MSDS

 25000

Protocol

 25000

Specifications

View the full PowerBiofilm^™ Application Note

PowerBiofilm Trailer

Storage Conditions

Lyophilized DNase I: 2 years at 4ºC.

All other reagents: 2 years at room temperature.

Publications

• Shun’ichi Ishii, Shino Suzuki, Trina M. Norden-Krichmar,w, Aaron Tenney, Patrick S.G. Chain, Matthew B. Scholz, Kenneth H. Nealson & Orianna Bretschger. A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer.. Nature Communications(2013) 4:1601 | DOI: 10.1038/ncomms2615

 

Kit Components