The BiOstic® FFPE Tissue RNA Isolation Kit is optimized for complete lysis and extraction of total RNA from formalin-fixed, paraffin-embedded tissues without the use of organic solvents. A proprietary buffer formulation in conjuction with a Proteinase K lysis facilitates complete dissolution of the wax during a 60°C heating step to release the tissue. A second 70°C heating step removes the cross-links that are inhibitory to RT-QPCR. RNA from the melted sample is purified on our new low elution silica spin filters to increase the final RNA concentration. Genomic DNA is removed in an on-column DNase digestion step utilizing our room temperature stable (RTS) DNase. RNA from the BiOstic® FFPE Tissue RNA Isolation Kit it ready to use in any downstream application including RT-QPCR, microarrays and sequencing.
|Format||Silica Spin Filter Tubes|
|Method||Direct melting of FFPE tissues|
|Sample Size||1 to 5 single slices or 15 mg of tissue|
|Binding Capacity||Up to 6 µg per spin filter|
|RNA Isolated||Total RNA|
|Equipment Required||Vortex, centrifuge, and water bath or heat block|
Store the Low Elution Spin Filters at 4°C.
Proteinase K (Solution FR2) and the RTS DNase are stable at room temperature for up to 6 months. For prolonged storage (up to 2 years), place the Solution FR2 and RTS DNase at 4°C.
Store all other reagents and kit components at room temperature (15-30°C).