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BiOstic® FFPE Tissue RNA Isolation Kit Sample

Overview

For the extraction of total RNA from FFPE tissue samples.
*Limit 1 per order
Order
Description Catalog # Price
2 Preps 13250-S $0.00    

Features

  •  Fast, safe paraffin removal - Avoids the use of xylene and other toxic solvents
  • Pure RNA - Contaminant-free RNA enables more sensitive results in RT-QPCR, microarrays and sequencing
  • Higher RNA concentration - New, low elution spin filter enables elution in a volume of just 20 µl

Description

 The BiOstic® FFPE Tissue RNA Isolation Kit is optimized for complete lysis and extraction of total RNA from formalin-fixed, paraffin-embedded tissues without the use of organic solvents.  A proprietary buffer formulation in conjuction with a Proteinase K lysis facilitates complete dissolution of the wax during a 60°C heating step to release the tissue.  A second 70°C heating step removes the cross-links that are inhibitory to RT-QPCR.  RNA from the melted sample is purified on our new low elution silica spin filters to increase the final RNA concentration.  Genomic DNA is removed in an on-column DNase digestion step utilizing our room temperature stable (RTS) DNase.  RNA from the BiOstic® FFPE Tissue RNA Isolation Kit it ready to use in any downstream application including RT-QPCR, microarrays and sequencing.

BiOstic® FFPE Tissue RNA Isolation Kit Sample
Click over image to enlarge

MSDS

 13250

Protocol

13250-S

Specifications

  

Format Silica Spin Filter Tubes
Method Direct melting of FFPE tissues
Sample Size 1 to 5 single slices or 15 mg of tissue
Binding Capacity Up to 6 µg per spin filter
RNA Isolated Total RNA
Throughput 1-24 samples
Time 50 minutes
Equipment Required Vortex, centrifuge, and water bath or heat block

Storage Conditions

Store the Low Elution Spin Filters at 4°C.

Proteinase K (Solution FR2) and the RTS DNase are stable at room temperature for up to 6 months.  For prolonged storage (up to 2 years), place the Solution FR2 and RTS DNase at 4°C.

Store all other reagents and kit components at room temperature (15-30°C).