The UltraClean® 6 Minute Mini Plasmid Prep Kit provides the fastest method and most efficient set of reagents available to purify plasmid DNA from 1 - 2 ml bacterial cultures. Based on a silica spin filter method, this kit elutes purified DNA in small working volumes of between 50 and 100 µl. See how easy it is to get pure plasmid DNA in just 6 minutes. This high quality DNA can be sequenced, used in restriction digests, ligation, PCR and other downstream applications. For a more traditional protocol, try the UltraClean® Standard Mini Plasmid Prep Kit.
A unique feature of this kit is its compatibility with high nutrient media. Low copy plasmid yield is improved by growth in high nutrient media; however, most competitor purification kits are not compatible with this media and can lead to genomic DNA contamination.
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Plasmids were isolated from 2ml of LB overnight cultures using either MO BIO Laboratories' Ultra Clean® 6 Minute Mini Plasmid Prep Kit or a kit from competitor Q.
Gel electrophoresis shows no difference in DNA yield or quality between the two kits.
There was no difference in the purity of the plasmid DNA, as demonstrated by A260/280 and A260/230 ratios.
PicoGreen was used to determine the concentration of dsDNA, revealing no difference in DNA yield between the two kits.
|Format||Silica Spin Filter Tubes|
|Binding Capacity||Up to 20 µg per filter|
|Throughput||1 - 24 samples|
Simple, effective and inexpensive kit for plasmid isolation
"The UltraClean® 6 Minute Mini Plasmid Prep kit allows the recovery of high yields of pure plasmid from E.coli strains. In our lab, we usually obtain plasmid DNA that gives an A260/A280 absorbance ratio of 1.8-1.9. The time spent for the isolation is less (6-10 minutes for each miniprep) than competitors, allowing multiple isolations to be performed subsequently (up to four preps without increasing the time)."...read the entire review at Biocompare.com
"I was very impressed with the MO BIO UltraClean® 6 Minute Mini Plasmid Prep Kit. I have used standard mini prep protocols and kits from other companies with variable results. The MO BIO kit gave clean, sequence ready DNA. The kit was user friendly, economical and fast. Less than $0.05/ug of clean plasmid DNA means it it less expensive than other leading brands and supplies plenty of plasmid for restriction digests, sequencing and other applications. Nice product."
UltraClean® 6 Minute Mini Plasmid Prep Kit
"I liked the kit. It is very fast, easy and simple to use. Plasmid was of good quality, worked for restriction digestion as well as transfections."
|1 x 3.3 ml
1 x 5.5 ml
1 x 18 ml
1 x 16.5 ml
1 x 3.3 ml
|1 x 6 ml
1 x 11 ml
1 x 34 ml
1 x 30 ml
1 x 6 ml
|1 x 15 ml
1 x 30 ml
1 x 90 ml
1 x 45 ml
1 x 15 ml