UltraClean® 6 Minute Mini Plasmid Prep Kit

Overview

Related Products

Features

- NEW: The UltraClean® 6 Minute Mini Plasmid Prep Kit has been updated to improve yield, purity and speed

- Rapid protocol yields high quality plasmid DNA in 6 minutes

- Easy format utilizes a silica spin filter method

- Robust yields up to 20μg of plasmid from 1-2 ml cultures

- Ideal for increasing yields of low copy plasmids grown in TB DRY^® Powder Growth Media

- Complete kit includes all silica spin filters and tubes

Description

The UltraClean^® 6 Minute Mini Plasmid Prep Kit provides the fastest method and most efficient set of reagents available to purify plasmid DNA from 1 - 2 ml bacterial cultures. Based on a silica spin filter method, this kit elutes purified DNA in small working volumes of between 50 and 100 µl. See how easy it is to get pure plasmid DNA in just 6 minutes. This high quality DNA can be sequenced, used in restriction digests, ligation, PCR and other downstream applications. For a more traditional protocol, try the UltraClean^® Standard Mini Plasmid Prep Kit.

A unique feature of this kit is its compatibility with high nutrient media. Low copy plasmid yield is improved by growth in high nutrient media; however, most competitor purification kits are not compatible with this media and can lead to genomic DNA contamination. As shown in the gel, the UltraClean^® 6 Minute Mini Plasmid Prep Kit increases yields of low copy plasmids, with no genomic DNA contamination in just 6 minutes vs. a leading competitor miniprep kit. 

Sample Kit

Click to try a sample.

Research and Development

Plasmids were isolated from 2ml of LB overnight cultures using either MO BIO Laboratories' Ultra Clean® 6 Minute Mini Plasmid Prep Kit or a kit from competitor Q.

Gel electrophoresis shows no difference in DNA yield or quality between the two kits.

There was no difference in the purity of the plasmid DNA, as demonstrated by A260/280 and A260/230 ratios.

PicoGreen was used to determine the concentration of dsDNA, revealing no difference in DNA yield between the two kits.

MSDS

12300

Protocol

12300

Specifications

Visual Protocol

Storage Conditions

Publications

• Caliante, S., Prisco, M., Sciarrillo, R., De Falco, M., Capaldo, A., Gay, G., Andreuccetti, P., Laforgia, V., and Varano, L.Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland . Journal of Endocrinology. 196 291-303 (2008).
• Lopez-Matas, M.A., Nuñez, P., Soto, A., Allona, I., Casado, R., Collada, C., Guevara, M.A., Aragoncillo, C., Gomez, L. Protein Cryoprotective Activity Of a Cytosolic Small Heat Shock Protein That Accumulates Constitutively In Chestnut Stems and Is Up-Regulated By Low and High Temperatures. Plant Physiology. 134(4): 1708-1717 (2004).
• Scott, H.N., Laible, P.D., Hanson, D.K. Sequences Of Versatile Broad-Host-Range Vectors Of The RK2 Family. Plasmid. 50(1): 74-79 (2003).

Customer Comments

Excellent Product
"I was very impressed with the MO BIO UltraClean^® 6 Minute Mini Plasmid Prep Kit. I have used standard mini prep protocols and kits from other companies with variable results. The MO BIO kit gave clean, sequence ready DNA. The kit was user friendly, economical and fast. Less than $0.05/ug of clean plasmid DNA means it it less expensive than other leading brands and supplies plenty of plasmid for restriction digests, sequencing and other applications. Nice product."

fowlkes@reed.edu

UltraClean^® 6 Minute Mini Plasmid Prep Kit
"I liked the kit. It is very fast, easy and simple to use. Plasmid was of good quality, worked for restriction digestion as well as transfections."

krupas@ncifcrf.gov
 

Kit Components