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UltraClean® Endotoxin-Free Ethanol Precipitation Kit
Overview
For concentrating endotoxin-free plasmid DNA by ethanol precipitation using guaranteed endotoxin-free reagentsOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 1 kit | 12616 | $141.00 |
|
Related Products
UltraClean® Endotoxin Removal KitEndotoxin-Free Reagents and Supplies
UltraClean® Endotoxin-Free Midi Plasmid Prep Kit
UltraClean® Endotoxin-Free Mini Plasmid Prep Kit
UltraClean® Endotoxin-Free Maxi Plasmid Prep Kit
Description
The UltraClean™ Endotoxin-Free Ethanol Precipitation Kit is designed to concentrate plasmid DNA that is already free of endotoxins. The plasmid DNA must be in a low salt solution. Plasmid DNA stored in a solution containing a high salt concentration should be diluted to 100 mM or less before using this kit.
Plasmid DNA is concentrated by adding 5M NaCl and Ethanol, incubating on ice and then centrifuging to obtain the DNA pellet. The precipitated plasmid DNA is then suitable for transfection, injection and other sensitive applications.
Endotoxins, also known as lipopolysaccharides, are cell membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. Endotoxins represent a non-controllable variable in transfection experiment setup, making results difficult to compare and interpret.
IMPORTANT NOTE: This kit does NOT remove endotoxins from DNA solutions.
To remove endotoxins from previously purified DNA, we recommend using the UltraClean™ Endotoxin Removal Kit.
Click over image to enlarge
MSDS
Protocol
Specifications
| Endotoxin Level | <0.1 EU/µg |
|---|---|
| Application | Transfection, injection |
| Contents |
All provided reagents are endotoxin-free |
Storage Conditions
Store at room temperature for up to 1 yearKit Components
| Component | 12616 |
|---|---|
| Endotoxin-Free Ethanol Endotoxin-Free Water Endotoxin-Free 5M NaCl |
2 x 30 ml 1 x 10 ml 1 x 10 ml |
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