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UltraClean® Plant RNA Isolation Kit
Overview
For isolation of total RNA from plant tissuesOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 20 preps | 13300-20 | $105.00 |
|
| 50 preps | 13300-50 | $237.00 |
|
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UltraClean® PCR Clean-Up KitPowerPlant® DNA Isolation Kit
UltraClean® -htp 96 Well Plant DNA Isolation Kit
Features
- Rapid isolation of total RNA from plant tissues in only 35 minutes
- Yields up to 60 μg high quality RNA for RT and RT-PCR
- Safe and easy protocol uses NO phenol/chloroform extraction, CsCl gradients or ethanol precipitation
- Includes all ready-to-use RNase-free reagents and all required spin filters and tubes
Description
The UltraClean® Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.
Fresh or frozen plant tissues are homogenized using standard manual methods. After cell lysis, the released RNA is captured on a silica membrane spin filter. The RNA bound to the filter is washed to remove all contaminants. Finally, the RNA is recovered in certified RNase-free water (provided). The eluted RNA is very pure and ready for sensitive applications such as reverse-transcription PCR.
Sample Kit
Click to try a sample.
Research and Development
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
UltraClean® Plant RNA Isolation Kit
and displayed on a denaturing formaldehyde agarose gel.
| Lane 1: Marker Lane 2: Spinach Lane 3: Onion |
Lane 4: Cauliflower Lane 5: Broccoli Lane 6: Radish |
Lane 7: Corn Lane 8: Sunflower Lane 9: Wheat Grass |
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Manual Homogenization |
| Starting Amount | 5-25mg |
| Binding Capacity | Up to 60µg per filter |
| Throughput | 1 - 24 samples |
| Time | 35 minutes |
UltraClean® Plant RNA Kit |
|||
| Spinach Radish Corn Grape Leaf |
Mint Alfalfa Tomato Grass |
Strawberry Wheat Rice Cowpea |
Lime Bean sprout Bell pepper Arabidopsis |
Storage Conditions
Store at room temperature for up to 2 years
Publications
- Larson-Rabin, Z., Li, Z., Masson, P.H., and Day, C.D. FZR2/CCS52A1 is a Determinant of Endoreduplication and Cell Expansion in 1 [W][OA]/. Plant Physiology. 149: 874-884 (2009).
- Mortimer-Jones, A.M., Jones, M.G.K., Jones, R.A.C., Thomas, G., and Dwyer, G.I. A Single Tube, Quantitative Real-time RT-PCR Assay that Detects Four Potato Viruses Simultaneously. Journal of Virological Methods. (2009).
- Tan, F.C., Swain, S.M. Functional Characterization Of AP3, SOC1 and WUS Homologues From Citrus (Citrus sinensis). Physiologia Plantarum. 131(3): 481-495 (2007).
Kit Components
| Component | 13300-20 | 13300-50 |
|---|---|---|
| Solution PMR1 Solution PMR2 Solution PMR3 Solution PMR4 Solution PMR5 Solution PMR6 Spin Filters in Tubes Collection Tubes |
1 x 25 ml 1 x 12 ml 1 x 8 ml 1 x 20 ml 1 x 15 ml 1 x 2 ml 20 40 |
1 x 60 ml 1 x 30 ml 1 x 15 ml 2 x 25 ml 1 x 30 ml 1 x 5 ml 50 100 |
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