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PowerLyzer™ UltraClean® Plant RNA Isolation Kit
Overview
For isolation of total RNA from plant tissues, optimized for use with bead based homogenizers such as the PowerLyzer™ 24.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 13355-50 | $237.00 |
|
Features
- Rapid isolation of total RNA from plant tissues in only 35 minutes
- Optimized for use with bead based homogenizers such as the PowerLyzer™ 24
- Yields up to 60 μg high quality RNA for RT and RT-PCR
- Safe and easy protocol uses NO phenol/chloroform extraction, CsCl gradients or ethanol precipitation
- Includes all ready-to-use RNase-free reagents and all required spin filters and tubes
Description
The PowerLyzer™UltraClean® Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.
Fresh or frozen plant tissues are homogenized using a bead based homogenizer like the PowerLyzer™ 24. After cell lysis, the released RNA is captured on a silica membrane spin filter. The RNA bound to the filter is washed to remove all contaminants. Finally, the RNA is recovered in certified RNase-free water (provided). The eluted RNA is very pure and ready for sensitive applications such as reverse-transcription PCR.
Sample Kit
Click to try a sample.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Based Homogenization |
| Starting Amount | 5-25mg |
| Binding Capacity | Up to 60µg per filter |
| Throughput | 1 - 24 samples |
| Time | 35 minutes |
Storage Conditions
Store at room temperature for up to 2 years
Kit Components
| Component | 13300-50 |
|---|---|
| Solution PMR1 Solution PMR2 Solution PMR3 Solution PMR4 Solution PMR5 Solution PMR6 Spin Filters in Tubes PowerLyzer™ Ceramic Bead Tubes Collection Tubes |
1 x 60 ml 1 x 30 ml 1 x 15 ml 2 x 25 ml 1 x 30 ml 1 x 5 ml 50 50 100 |
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