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UltraClean® -htp 96 Well Plant DNA Isolation Kit
Overview
For high throughput isolation of DNA from plant tissuesOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 13096-4 | $950.00 |
|
| 12 x 96 preps | 13096-12 | $2,600.00 |
|
Features
- Process 96 plant tissue samples in under 3 hours
- Removes polysaccharide and phenolic inhibitors of PCR
- Isolates pure DNA up to 50kb plus in length
- No hazardous organic solvents for lab safety
Description
The UltraClean®-htp 96 Well Plant DNA Isolation Kit provides high-throughput isolation of high-quality DNA from 96 samples in less than 3 hours. The simple 96 well procedure provides highly reproducible yields of total cellular DNA. The streamlined procedure and convenient 96 well format eliminate tedious labeling, one-at-a-time pipetting and DNA resuspension steps, and help minimize cross-contamination. Polysaccharides, proteins and enzyme inhibitors are efficiently removed, making the purified DNA suitable for use in all downstream applications such as PCR.
Without the use of organic solvents like phenol and chloroform, these kits will safely isolate genomic DNA from plant tissues. Plant tissue samples are added to a 96 well plate containing beads, bead solution and lysis solution. The tissue is lysed by mechanical action on a 96 Well Plate Shaker. The lysate is centrifuged through a spin plate containing a silica membrane. Under high salt conditions, the DNA is bound to the silica membrane. The spin plate is washed to remove any contaminants and the DNA is finally eluted into certified DNA-free Tris buffer.
Research and Development
 |
| -PCR Product |
| -primers |
performed on plant DNA isolated with the
UltraClean®-htp 96 Well Plant DNA Isolation Kit.
| Lane 1: DNA Marker Lane 2: Spinach Lane 3: Cowpea Lane 4: Radish Lane 5: Bell Pepper |
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Plate |
|---|---|
| Method | Bead Beating |
| Starting Amount | 5-50 mg |
| Binding Capacity | Up to 40 µg per well |
| Throughput | 96 samples |
| Time | under 3 hours |
UltraClean-htp™ 96 Well Plant DNA Isolation Kit |
|||
| Spinach Radish Corn Grape Leaf |
Mint Alfalfa Tomato Grass |
Strawberry Wheat Rice Cowpea |
Lime Bean sprout Bell pepper Arabidopsis |
Storage Conditions
Store at room temperature for up to 2 years.Publications
- Kardaru, S., Zhang, W., Yadav, A., Oard, J.H. Development and Application Of Allele-Specific PCR Assays For Imazethapyr Resistance in rice (Oryza sativa). Euphytica. 160(3): 431-438 (2008).
Kit Components
| Component | 13096-4 | 13096-12 |
|---|---|---|
| Bead Plates Bead Solution Solution P1 Solution P2 Solution P3 Solution P4 Solution P5 Spin Plates Filter Plates 2 ml Collection Plates 1 ml Collection Plates 0.5 ml Collection Plates Microplates Centrifuge Tape Sealing Tape Elution Sealing Mat |
4 1 x 233 ml 1 x 26 ml 1 x 106 ml 1 x 592 ml 12 x 30 ml 1 x 43 ml 4 4 8 4 8 4 36 8 4 |
12 1 x 699 ml 1 x 78 ml 1 x 318 ml 1 x 1776 ml 36 x 30 ml 1 x 129 ml 12 12 24 12 24 12 108 24 12 |
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