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PowerPlant® Pro-htp 96 Well DNA Isolation Kit

Overview

For high-throughput isolation of genomic DNA from plant and seed samples, including those high in polyphenolics and polysaccharides.
Order
Description Catalog # Price
4 x 96 preps 13496-4 $1,165.00    
4 x 96 preps including bead plates 13496-BUNDLE $1,340.00    

Features

  • High-throughput method - Isolate high quality, pure DNA from plant samples in a 96 well format
  • Optimized for tough samples - Isolate DNA from the most difficult plant types, including strawberry leaf, cotton leaf, cotton seeds and pine needles
  • Inhibitor Removal Technology® - Removes polyphenolics, polysaccharides and other PCR inhibitors
  • Phenolic Separation Solution - Breaks the bond between DNA and phenolics, increasing DNA yield in high-phenolic samples

Description

Isolate pure, high quality DNA from the toughest plant samples

The PowerPlant® Pro-htp 96 Well DNA Isolation Kit is designed for high-throughput purification of genomic DNA from plant cells, tissues and seeds. The optimized protocol replaces time-consuming DNA isolation procedures such as CTAB, phenol, or chloroform extraction for recovery of high quality DNA from the toughest sample types, including strawberry leaf, cotton leaf, cotton seeds, and pine needles.

Patented Inhibitor Removal Technology® (IRT) is used for removal of PCR inhibitors from plant extracts during the isolation process, resulting in pure DNA that is ready to use in downstream applications including PCR, QPCR and sequencing. In addition, a unique Phenolic Separation Solution (PSS) is included as an optional step for samples high in polyphenolic compounds, such as pine and grape leaf. PSS breaks the bond between DNA and phenolics, preventing loss of DNA during the IRT step and increasing DNA yield.

The PowerPlant® Pro-htp 96 Well DNA Isolation Kit is available with (13496-BUNDLE) or without (13496-4) Metal Bead Plates, which can be used for optional bead-based homogenizaiton. Alternatively, another bead plate or method of choice may be used to break down samples. Following an inhibitor removal step, total genomic DNA is captured on a 96 well silica membrane in a spin filter format. DNA is then washed and eluted from the spin filter plate. DNA is ready to use in PCR, qPCR or next generation sequencing.

Sample Kit

For protocol optimization, try our PowerPlant® Pro DNA isolation Kit, which uses the same chemistry as the PowerPlant® Pro-htp 96 Well DNA Isolation Kit. 

Click to try a sample

Research and Development

 

Figure 1. Isolation of high quality genomic DNA from plant samples using the PowerPlant® Pro DNA Isolation Kit. DNA isolated from grass (2 ul), strawberry  leaf (15 ul), grape leaf (15 ul) and pine needles (1 ul) was examined on a 1% TAE agarose gel. 

Figure 2. The PowerPlant® Pro DNA isolation kit provides greater yields of high molecular weight genomic DNA. DNA was isolated from cotton seed using the PowerPlant® Pro DNA Isolation Kit and plant DNA isolation kits from three other suppliers. All isolations were performed according to the manufacturer’s protocol. DNA (2% of elution volume) was examined on a 1% TAE agarose gel.

 

 Figure 3. Successful amplification of DNA isolated using the PowerPlant® Pro DNA Isolation Kit. QPCR was performed using primers for RuBisCo on 1 ng of DNA isolated from pine needles (A) and strawberry leaf (B). Undiluted DNA and samples diluted 1:10 both fell within the standard curve (purple lines), and the difference between the diluted and undiluted samples was approximately 3 cycles.

 

 

 

PowerPlant® Pro-htp 96 Well DNA Isolation Kit
Click over image to enlarge

MSDS

13496-4

Protocol

13496-4

Specifications

Format Silica Spin Filter Plates
Starting Amount Up to 50 mg
Binding Capacity Up to 40 µg per filter
Throughput 96-384 samples
Time Process up to 384 samples in less than one day
Equipment Required

96 Well Plate Shaker

Centrifuge

Metal Bead Plates are included with 13496-BUNDLE.

Storage Conditions

RNase A should be stored at 4°C for up to 2 years

The other kit reagents and components should be stored at room temperature (15-30°C) for up to 2 years

Kit Components

Component 13496-4 13496-BUNDLE
Solution PD1 200 ml 200 ml
Solution PD2 22 ml 22 ml
Solution PD3 106 ml 106 ml
Solution PD4 255 ml 255 ml
Solution PD5 106 ml 106 ml
Solution PD7 43 ml 43 ml
RNase A Solution 1.5 ml 1.5 ml
Phenolic Separation Solution 17 ml 17 ml
Spin Plates 4 plates 4 plates
2 ml Collection Plates 4 plates 4 plates
1 ml Collection Plates 4 plates 4 plates
0.5 ml Collection Plates 4 plates 4 plates
Microplates 4 plates 4 plates
Centrifuge Tape 48 tapes 48 tapes
Sealing Tape 16 tapes 16 tapes
Elution Sealing Mats 4 mats 4 mats
Metal Bead Plates Not included 4 plates