|4 x 96 preps||13496-4||$1,165.00|
|4 x 96 preps including bead plates||13496-BUNDLE||$1,340.00|
Isolate pure, high quality DNA from the toughest plant samples
The PowerPlant® Pro-htp 96 Well DNA Isolation Kit is designed for high-throughput purification of genomic DNA from plant cells, tissues and seeds. The optimized protocol replaces time-consuming DNA isolation procedures such as CTAB, phenol, or chloroform extraction for recovery of high quality DNA from the toughest sample types, including strawberry leaf, cotton leaf, cotton seeds, and pine needles.
Patented Inhibitor Removal Technology® (IRT) is used for removal of PCR inhibitors from plant extracts during the isolation process, resulting in pure DNA that is ready to use in downstream applications including PCR, QPCR and sequencing. In addition, a unique Phenolic Separation Solution (PSS) is included as an optional step for samples high in polyphenolic compounds, such as pine and grape leaf. PSS breaks the bond between DNA and phenolics, preventing loss of DNA during the IRT step and increasing DNA yield.
The PowerPlant® Pro-htp 96 Well DNA Isolation Kit is available with (13496-BUNDLE) or without (13496-4) Metal Bead Plates, which can be used for optional bead-based homogenizaiton. Alternatively, another bead plate or method of choice may be used to break down samples. Following an inhibitor removal step, total genomic DNA is captured on a 96 well silica membrane in a spin filter format. DNA is then washed and eluted from the spin filter plate. DNA is ready to use in PCR, qPCR or next generation sequencing.
For protocol optimization, try our PowerPlant® Pro DNA isolation Kit, which uses the same chemistry as the PowerPlant® Pro-htp 96 Well DNA Isolation Kit.
Click to try a sample
Figure 1. Isolation of high quality genomic DNA from plant samples using the PowerPlant® Pro DNA Isolation Kit. DNA isolated from grass (2 ul), strawberry leaf (15 ul), grape leaf (15 ul) and pine needles (1 ul) was examined on a 1% TAE agarose gel.
Figure 2. The PowerPlant® Pro DNA isolation kit provides greater yields of high molecular weight genomic DNA. DNA was isolated from cotton seed using the PowerPlant® Pro DNA Isolation Kit and plant DNA isolation kits from three other suppliers. All isolations were performed according to the manufacturer’s protocol. DNA (2% of elution volume) was examined on a 1% TAE agarose gel.
Figure 3. Successful amplification of DNA isolated using the PowerPlant® Pro DNA Isolation Kit. QPCR was performed using primers for RuBisCo on 1 ng of DNA isolated from pine needles (A) and strawberry leaf (B). Undiluted DNA and samples diluted 1:10 both fell within the standard curve (purple lines), and the difference between the diluted and undiluted samples was approximately 3 cycles.
|Format||Silica Spin Filter Plates|
|Starting Amount||Up to 50 mg|
|Binding Capacity||Up to 40 µg per filter|
|Time||Process up to 384 samples in less than one day|
Metal Bead Plates are included with 13496-BUNDLE.
RNase A should be stored at 4°C for up to 2 years
The other kit reagents and components should be stored at room temperature (15-30°C) for up to 2 years
|Solution PD1||200 ml||200 ml|
|Solution PD2||22 ml||22 ml|
|Solution PD3||106 ml||106 ml|
|Solution PD4||255 ml||255 ml|
|Solution PD5||106 ml||106 ml|
|Solution PD7||43 ml||43 ml|
|RNase A Solution||1.5 ml||1.5 ml|
|Phenolic Separation Solution||17 ml||17 ml|
|Spin Plates||4 plates||4 plates|
|2 ml Collection Plates||4 plates||4 plates|
|1 ml Collection Plates||4 plates||4 plates|
|0.5 ml Collection Plates||4 plates||4 plates|
|Microplates||4 plates||4 plates|
|Centrifuge Tape||48 tapes||48 tapes|
|Sealing Tape||16 tapes||16 tapes|
|Elution Sealing Mats||4 mats||4 mats|
|Metal Bead Plates||Not included||4 plates|