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PowerPlant® DNA Isolation Kit
Overview
For isolating quality DNA from plants including those high in phenolics and polysaccharides.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 13200-50 | $165.00 |
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| 100 preps | 13200-100 | $303.00 |
|
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Features
- New method improves PCR inhibitor removal
- Included steel beads enhance sample homogenization
- Utilizes easy hands-off homogenization
- Homogenization method eliminates cross-contamination and the need to clean grinding tools between samples
- Includes tough customized tubes to bead beat and homogenize without tube breakage
- Process more samples with less clean up
- Compatible with vortexes, MO BIO’s PowerLyzer™ 24 or any bench top homogenizer
Description
The PowerPlant® DNA Isolation Kit isolates genomic DNA free from PCR impurities from even the toughest plant samples using a powerful homogenization method. Due to the unique hands-off bead beating method, up to 24 samples can be processed simultaneously using a vortex adapter or a bench top homogenizer, such as our PowerLyzer™ 24 Homogenizer. This method effectively eliminates both bottlenecks and cross-contamination risk found with hand grinding with a mortar and pestle.
The PowerPlant® DNA Isolation Kit saves significant time when compared to traditional liquid nitrogen methods and yields are comparable. In addition, its novel PCR inhibitor removal system ensures the DNA is free of polyphenols and polysaccharides allowing for all downstream applications including successful PCR analysis.
Powerful Protocol
The PowerPlant® DNA Isolation Kit protocol begins by adding plant material to the provided tubes containing specialized beads along with optimized reagents for rapid homogenization. Cell lysis and DNA release occur by both mechanical and chemical methods to maximize DNA isolation efficiency. A silica membrane in a spin column format is used to capture, wash and elute the pure DNA.
The PowerPlant® DNA Isolation Kit has been successfully utilized on a wide variety of plant types including leaf, roots, and seed samples. Occasionally, plants such as pine needles, cotton, sunflower, and strawberry present additional challenges and require an additional clean up procedure. The PowerPlant® DNA Isolation Kit provides special clean-up reagents as an optional step to assure success among even the most challenging plant samples.
Sample Kit
Click to try a sample.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Starting Amount | 50 mg |
| Binding Capacity | Up to 40 µg per filter |
| Throughput | 1 - 24 samples |
| Time | 60 minutes |
| Equipment Required | Vortex and Vortex Adapter |
Storage Conditions
Store at room temperature for up to 2 years.Publications
- Samuels, G.J., Lu, B-S., Chaverri, P., Candoussau, F., Fournier, J., and Rossman, A.Y. Cyanonextria ad New Genus for Nectria cyanostoma and its Fusarium Anamorph. Mycological Progress. 8(1): 49-58 (2009).
- Desalvo, M.K., Voolstra, C.R., Sunagawa, S., Schwarz, J.A., Stillman, J.H., Coffroth, M.A., Szmant, A.M., and Medina, M. Differential gene expression during thermal stress and bleaching in the Caribbean coral Montastraea faveolata. Molecular Ecology. 17: 3952-3971 (2008).
Kit Components
| Component | 13200-50 | 13200-100 |
|---|---|---|
| Bead Tubes Bead Solution Solution PB1 Solution PB2 Solution PB3 Solution PB4 Solution PB5 Solution PB6 Solution PB7 Spin filters in Tubes 2 ml Collection Tubes 2.2 ml Collection Tubes |
50 30 ml 5.5 ml 20 ml 3 x 30 ml 28 ml 30 ml 11 ml 4 ml 50 100 50 |
100 70 ml 11 ml 43 ml 6 x 30 ml 55 ml 2 x 30 ml 22 ml 8 ml 100 200 100 |
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