|4 x 96 preps||27700-4-KF||$1,500.00|
|4 x 96 preps including bead plates||27700-BUNDLE||$1,675.00|
The PowerMag® Seed DNA Isolation Kit is designed for fast and easy purification of nucleic acids from all seed types. The optimzed bead beating technology (optional) replaces cumbersome isolation procedures such as CTAB, phenol, or chloroform extraction for recovery of high quality nucleic acids from the toughest seed types, including flax, wheat, canola, corn and barley. Patented Inhibitor Removal Technology® (IRT) is used for removal of PCR inhibitors from seed extracts during the isolation process. Additionally, a unique Phenolic Separation Solution (PSS) is included as an optional step for seeds high in polyphenolic compounds, such as cotton, watermelon, and bell pepper seeds. PSS breaks the bond between nucleic acids and phenolics, increasing the DNA yield.
The PowerMag® Seed DNA Isolation Kit is available with (27700-BUNDLE) or without (27700-4-KF) Metal Bead Plates, which can be used for optional bead-based homogenizaiton. Alternatively, another bead plate or method of choice may be used to break down samples. Novel ClearMag® technology enables purification of DNA without the typical surface binding to the beads, eliminating the adsorption of organic inhibitors that is typical of other magnetic bead technologies, and facilitating isolation of pure DNA. In addition, neither chaotropic salts nor ethanol are used in the binding and washing steps, removing a second source of contamination that can inhibit enzymatic reactions. The PowerMag® Seed DNA Isolation Kit has been optimized for use with the Thermo Scientific KingFisher® Flex and KingFisher® Duo automated processing systems, for rapid, high-throughput isolation of inhibitor-free DNA. The high-quality DNA isolated with the PowerMag® Seed DNA Isolation Kit is ready to use in PCR, qPCR and next generation sequencing.
Figure 1. High quality, pure DNA was isolated from duplicate samples of flax seeds (25 mg with seed coat removed), wheat seeds (45 mg), canola seeds (10 whole seeds, 50 mg), corn kernels (50 mg), and barley seeds (50 mg) using the PowerMag® Seed DNA Isolation Kit. High quality, high molecular weight DNA was observed on a 1.2% TAE agarose gel. No differences in yield or quality were observed between the duplicate samples. DNA yields are shown in Table 1. Lane 1: Marker; Lanes 2-3: Flax; Lanes 4-5: Wheat; Lanes 6-7: Canola; Lanes 8-9: corn; Lanes 10-11: Barley; Lane 12: Marker.
|Table 1. High yields of DNA isolated from seeds.|
|Figure 2. Successful amplification of DNA isolated from flax seeds using the PowerMag® Seed DNA Isolation Kit. qPCR was performed on duplicate samples using the Universal Plant (Rubisco) assay. Undiluted DNA (1 µl of elution, dark blue lines) and samples diluted 1:10 (light blue lines) fell within the standard curve (grey lines), and the difference between the diluted and undiluted samples was approximately 3 cycles, indicating the DNA was free of inhibitors. There was no difference in Cq value between the duplicate samples.|
|Starting Amount||25 to 50 mg|
|Throughput||96 well plate|
Metal Bead Plates are included with 27700-BUNDLE.
PowerMag™ Seed Flyer
|PowerMag® Bead Solution||200 ml||200 ml|
|PowerMag® Lysis Solution||26 ml||26 ml|
|PowerMag® IRT Solution||106 ml||106 ml|
|Phenolic Separation Solution||17 ml||17 ml|
|ClearMag® Binding Solution||200 ml||200 ml|
|ClearMag® Beads||9 ml||9 ml|
|ClearMag® Wash Solution||640 ml||640 ml|
|ClearMag® Elution Buffer||44 ml||44 ml|
|RNase A Solution||2 x 1 ml (25mg/ml)||2 x 1 ml (25mg/ml)|
|PowerMag® 1 ml Collection Plates||8 plates||8 plates|
|Sealing Tape||32 tapes||32 tapes|
|Metal Bead Plates||Not Included||4 plates|