MO BIO's Exhibitor Seminar at ASM 2010

Yield, Quality and Purity:
Considerations for DNA Extraction from Complex Microbiological Samples

Guest Speaker: Janet K. Jansson, Ph.D., Professor and Senior Staff Scientist, Molecular Microbial Ecology Group, Earth Science Division, Lawrence Berkeley National Laboratory

When: May 24th, 7:00pm

Where: Manchester Grand Hyatt, San Diego, CA
1 Market Place
San Diego, CA 92101

Hors d'oeuvre and 1 free drink will be served.

Synopsis

Yield, Quality and Purity: Considerations for DNA Extraction from Complex Microbiological Samples:

Problems achieving high yields and purity DNA are exaggerated in microbial samples, and especially those from complex environments (ex. soil, biofilms, gut) because of the variety of tough to lyse microorganisms and the high level of enzymatic inhibitors that co-purify with nucleic acids.

Successful microbial community analysis depends on high efficiency extraction and purification that accurately reflects the quantity of DNA or RNA in a sample. Environmental samples can have extreme differences between neighboring collection sites, collection depths, or even between individual gut microbiota, increasing the challenge of accurate profiling of different ecological habitats. This workshop will discuss considerations for obtaining high and accurate yields and purity of DNA from various samples isolated from environmental sources. The introduction will provide an overview of some popular misconceptions to watch for when analyzing DNA. Janet Jansson will present her work that focuses on the analysis of microbial ecology of diverse sample types and the importance of DNA yield, quality and purity in research.

Attention, space is limited! There are only 200 spots available. Make sure to register in advance to ensure your spot: simply send an email with your name and company name to info@mobio.com, with "ASM Seminar" as a subject line.