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UltraClean® Microbial RNA Isolation Kit

Overview

For isolation of total RNA from microorganisms.
Order
Description Catalog # Price
50 preps 15800-50 $215.00    

Features

  •  Isolates total RNA from microbial cells in 35 minutes
  • Yields high quality RNA ready for qPCR
  • Complete kit includes all required RNase-free tubes and ready to use reagents
  • Ideal for Gram positive and Gram negative bacteria, yeast, fungi and bacterial spores

Description

The UltraClean® Microbial RNA Isolation Kit is designed to yield up to 60 µg of high-quality RNA from pure cultures of microorganisms in 35 minutes. Based on a bead beating technology, cells are lysed, RNA is captured on a silica spin filter, washed and eluted in a certified RNase-free elution buffer, ready for reverse transcription and RT-PCR. This kit includes all required RNase-free tubes and reagents ready-to-use. Requires our Vortex Adapter for maximum efficiency. This kit does not require organic solvents and is considered safer than TRIZOL®- based protocols.

Sample Kit

Click to try a sample.

Research and Development

High Quality Total RNA from Bacteria and Yeast
 
  1 2 3 4
 

Total RNA was isolated from various microorganisms and
displayed on a 1% formaldehyde/MOPS agarose gel.

        Lane 1: RNA Ladder
        Lane 2: E. faecalis (23S & 16S)
        Lane 3: B. subtilis (23S & 16S)
        Lane 4: C. albicans (28S & 18S)
UltraClean® Microbial RNA Isolation Kit
Click over image to enlarge

MSDS

15800

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 60 µg per filter
Throughput 1 - 24 samples
Time 35 minutes

 

Microorganisms tested
with UltraClean Kit
  • B. subtilis
  • B. pumulis
  • E. coli
  • E. faecalis
  • P. aeruginosa
  • M. luteus
  • S. aureus
  • L. casei
  • A. niger
  • C. albicans
  • S. cerevisiae

Storage Conditions

Store Solution MR2 at 4°C. Store other components at room temperature for up to 2 years.

Publications

Kit Components

Component 15800-50 15800-250
Solution MR1
Solution MR2
Solution MR3
Solution MR4
Solution MR5
Bead Tubes
RNase free Water
Spin Filters In Tubes
Collection Tubes
1 x 16 ml
1 x 850 μl
1 x 28 ml
1 x 15 ml
1 x 32 ml
50
1 x 5 ml
50
200
1 x 80 ml
1 x 4 ml
1 x 130 ml
1 x 65 ml
3 x 33 ml
250
1 x 15 ml
250
1000