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UltraClean® Microbial RNA Isolation Kit

Overview

For isolation of total RNA from microorganisms.
Order
Description Catalog # Price
50 preps 15800-50 $215.00    

Features

  •  Isolates total RNA from microbial cells in 35 minutes
  • Yields high quality RNA ready for qPCR
  • Complete kit includes all required RNase-free tubes and ready to use reagents
  • Ideal for Gram positive and Gram negative bacteria, yeast, fungi and bacterial spores

Description

The UltraClean® Microbial RNA Isolation Kit is designed to yield up to 60 µg of high-quality RNA from pure cultures of microorganisms in 35 minutes. Based on a bead beating technology, cells are lysed, RNA is captured on a silica spin filter, washed and eluted in a certified RNase-free elution buffer, ready for reverse transcription and RT-PCR. This kit includes all required RNase-free tubes and reagents ready-to-use. Requires our Vortex Adapter for maximum efficiency. This kit does not require organic solvents and is considered safer than TRIZOL®- based protocols.

Sample Kit

Click to try a sample.

Research and Development

High Quality Total RNA from Bacteria and Yeast
 
  1 2 3 4
 

Total RNA was isolated from various microorganisms and
displayed on a 1% formaldehyde/MOPS agarose gel.

        Lane 1: RNA Ladder
        Lane 2: E. faecalis (23S & 16S)
        Lane 3: B. subtilis (23S & 16S)
        Lane 4: C. albicans (28S & 18S)
UltraClean® Microbial RNA Isolation Kit
Click over image to enlarge

MSDS

15800

Protocol

15800

 

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 60 µg per filter
Throughput 1 - 24 samples
Time 35 minutes

 

Microorganisms tested
with UltraClean Kit
  • B. subtilis
  • B. pumulis
  • E. coli
  • E. faecalis
  • P. aeruginosa
  • M. luteus
  • S. aureus
  • L. casei
  • A. niger
  • C. albicans
  • S. cerevisiae

Storage Conditions

Store Solution MR2 at 4°C. Store other components at room temperature for up to 2 years.

Publications

Kit Components

Component 15800-50 15800-250
Solution MR1
Solution MR2
Solution MR3
Solution MR4
Solution MR5
Bead Tubes
RNase free Water
Spin Filters In Tubes
Collection Tubes
1 x 16 ml
1 x 850 μl
1 x 28 ml
1 x 15 ml
1 x 32 ml
50
1 x 5 ml
50
200
1 x 80 ml
1 x 4 ml
1 x 130 ml
1 x 65 ml
3 x 33 ml
250
1 x 15 ml
250
1000