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UltraClean® Microbial RNA Isolation Kit
Overview
For isolation of total RNA from microorganismsOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 15800-50 | $200.00 |
|
| 250 preps | 15800-250 | $929.00 |
|
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PowerMicrobial® Midi DNA Isolation KitUltraClean® Microbial DNA Isolation Kit
PowerMicrobial® Maxi DNA Isolation Kit
UltraClean® -htp 96 Well Microbial DNA Kit
Features
- Isolates total RNA from microbial cells in 35 minutes
- Yields up to 60 μg high quality RNA for RT and RT-PCR.
- Complete kit includes all required RNase-free tubes and ready to use reagents
- Ideal for gram + and gram - bacteria, yeast, fungi and bacterial spores
Description
The UltraClean® Microbial RNA Isolation Kit is designed to yield up to 60 µg of high-quality RNA from pure cultures of microorganisms in 35 minutes. Based on a bead beating technology, cells are lysed, RNA is captured on a silica spin filter, washed and eluted in a certified RNase-free elution buffer, ready for reverse transcription and RT-PCR. This kit includes all required RNase-free tubes and reagents ready-to-use. Requires our Vortex Adapter for maximum efficiency. This kit does not require organic solvents and is considered safer than TRIZOL®- based protocols.
Sample Kit
Click to try a sample.
Research and Development
| 1 | 2 | 3 | 4 |
Total RNA was isolated from various microorganisms and
displayed on a 1% formaldehyde/MOPS agarose gel.
- Lane 1: RNA Ladder
Lane 2: E. faecalis (23S & 16S)
Lane 3: B. subtilis (23S & 16S)
Lane 4: C. albicans (28S & 18S)
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 60 µg per filter |
| Throughput | 1 - 24 samples |
| Time | 35 minutes |
with UltraClean Kit |
|
|
|
Storage Conditions
Store Solution MR2 at 4°C. Store other components at room temperature for up to 2 years.
Publications
- Toh, S.M., Xiong, L., Bae, T., Mankin, A.S. The Methyltransferase YfgB/RlmN Is Responsible For Modification Of Adenosine 2503 In 23S rRNA. RNA. 14(1): 98-106 (2008).
- Poulain, A.J., Ní Chadhain, S.M., Ariya, P.A., Amyot, M., Garcia, E., Campbell, P.G., Zylstra, G.J., Barkay, T. Potential For Mercury Reduction By Microbes In The High Arctic. Applied and Environmenal Microbiology. 73(7): 2230-2238 (2007).
- Toh, S.M., Xiong, L., Arias, C.A., Villegas, M.V., Lolans, K., Quinn, J., Mankin, A.S. Acquisition Of A Natural Resistance Gene Renders A Clinical Strain Of Methicillin-Resistant Staphylococcus aureus Resistant To the Synthetic Antibiotic Linezolid. Molecular Microbiology. 64(6): 1506-1514 (2007).
Kit Components
| Component | 15800-50 | 15800-250 |
|---|---|---|
|
Solution MR1 Solution MR2 Solution MR3 Solution MR4 Solution MR5 Bead Tubes RNase free Water Spin Filters In Tubes Collection Tubes |
1 x 16 ml 1 x 850 μl 1 x 28 ml 1 x 15 ml 1 x 32 ml 50 1 x 5 ml 50 200 |
1 x 80 ml 1 x 4 ml 1 x 130 ml 1 x 65 ml 3 x 33 ml 250 1 x 15 ml 250 1000 |
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