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UltraClean® Microbial DNA Isolation Kit

Overview

For isolation of high quality DNA from microbial cultures.
Order
Description Catalog # Price
50 preps 12224-50 $140.00    
250 preps 12224-250 $563.00    

Features

  •  Rapidly purifies DNA from microbial cultures in 20 minutes
  • Yields high quality and high molecular weight DNA is ready for PCR, restriction digests and other downstream applications
  • Isolates DNA from yeast, fungi, Gram-negative and Gram-positive bacteria
  • No hazardous organic solvents or enzymes required

Description

The UltraClean® Microbial DNA Isolation kit is designed to isolate high quality genomic DNA from 1.8 ml of microbial culture. A variety of microorganisms including bacterial spores and fungal types have been tested successfully with this kit.

The principal of this kit is to lyse the microorganisms by a combination of heat, detergent, and mechanical force against specialized beads. The cells are lysed using our specially designed Vortex Adapter on a standard vortex. The released DNA is then bound to a silica spin filter, washed and the DNA is recovered in certified DNA-free Tris buffer. The UltraClean® Microbial DNA Isolation Kit requires our Vortex Adapter for maximum cell lysis efficiency.

 

Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System! 

Sample Kit

Click to try a sample.

Research and Development

High-Quality DNA Purified from Various Microorganisms Using the UltraClean® Microbial DNA Isolation Kit

      1 2 3 4 5 6 7 8 9 

   10 11 12 13 14                    
Lane 1: 1 kb ladder
Lane 2: E. coli
Lane 3: P. aeruginosa
Lane 4: S. aureus
Lane 5: M. luteus
Lane 6: B. subtilis
Lane 7: B. pumulis
Lane 8: E. faecalis
Lane 9: L. casei
Lane 10: 1 kb ladder
Lane 11: A. niger
Lane 12: C. albicans
Lane 13: S. cerevisiae
Lane 14: S. cerevisiae 

Analysis displayed on 1.2% TAE agarose gel. Lower yields obtained with A. niger (11) and C. albicans (12) due to lower cell densities in starting culture.

 

UltraClean® Microbial DNA Isolation Kit
Click over image to enlarge

MSDS

12224

Protocol

12224

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Binding Capacity Up to 20 µg per filter
Through-put 1 - 24 samples
Time 20 minutes
Microorganisms tested
with UltraClean® Kit
  • B. subtilis
  • B. pumulis
  • E. coli
  • E. faecalis
  • P. aeruginosa
  • M. luteus
  • S. aureus
  • L. casei
  • A. niger
  • C. albicans
  • S. cerevisiae

 

Storage Conditions

Store at room temperature for up to 2 years.

Publications

Customer Comments

Easy, 30 minute purification of DNA from microbes

"Overall, the MoBio Laboratories UltraClean Microbial DNA isolation kit is quick, easy and affordable. It has found a method to overcome the lysing issues faced by researchers who work with gram positive and/or resistant organisms. I am pleased with this kit and would highly recommend it to others."...read the entire review at Biocompare.com

 

New Method for Ectomycorrhizal Roots

The MO BIO UltraClean® Microbial DNA Kit works well for us. We have been trying to isolate DNA from ectomycorrhizal roots. Published methods for this are available, but they are slow and may involve highly toxic chemicals. When isolating DNA from pine ectomycorrhizas, we use the Microbial DNA Kit in conjunction with IRS (Inhibitor Removal Solution, available from MO BIO) and obtain PCR-ready DNA. No grinding or freezing is necessary. The process is simple and rapid.

Roger Koide
The Pennsylvania State University

Kit Components

Component 12224-50 12224-250
Solution MD1
Solution MD2
Solution MD3
Solution MD4
Solution MD5
Microbead Solution
Microbead Tubes
Spin Filters In Tubes
Collection Tubes
1 x 2.75 ml
1 x 5.5 ml
1 x 50 ml
1 x 16.5 ml
1 x 7 ml
50
1 x 16.5 ml
50
200
1 x 15 ml
1 x 30 ml
1 x 250 ml
3 x 25 ml
1 x 15 ml
250
1 x 80 ml
250
1000