UltraClean® Microbial DNA Isolation Kit

Overview

Related Products

Features

•  Rapidly purifies DNA from microbial cultures in 20 minutes

• Yields high quality DNA up to 50 kb plus that is ready for PCR, restriction digests and other downstream applications

• Isolates DNA from yeast, fungi, Gram-negative and Gram-positive bacteria

• No hazardous organic solvents or enzymes required

Description

The UltraClean^® Microbial DNA Isolation kit is designed to isolate high quality genomic DNA from 1.8 ml of microbial culture. A variety of microorganisms including bacterial spores and fungal types have been tested successfully with this kit.

The principal of this kit is to lyse the microorganisms by a combination of heat, detergent, and mechanical force against specialized beads. The cells are lysed using our specially designed Vortex Adapter on a standard vortex. The released DNA is then bound to a silica spin filter, washed and the DNA is recovered in certified DNA-free Tris buffer. The UltraClean^® Microbial DNA Isolation Kit requires our Vortex Adapter for maximum cell lysis efficiency.

 NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System! 

Sample Kit

Click to try a sample.

Research and Development

High-Quality DNA Purified from Various Microorganisms Using the UltraClean^® Microbial DNA Isolation Kit

MSDS

12224

Protocol

12224

Specifications

Storage Conditions

Store at room temperature for up to 2 years.

Publications

• L. Quigley, O. O’Sullivan, T.P. Beresford, R. Paul Ross, G.F. Fitzgerald and P.D. Cotter. A comparison of methods used to extract bacterial DNA from raw milk and raw milk cheese. Journal of Applied Microbiology, 24 APR 2012; 113(1): 96-105.
• Johana Husserl, Joseph B. Hughes, and Jim C. Spain.  Key Enzymes Enabling the Growth of Arthrobacter sp. Strain JBH1 with Nitroglycerin as the Sole Source of Carbon and Nitrogen. Appl. Envir. Microbiol., May 2012; 78: 3649 – 3655.
• Kathryn A. Harris, Dervla T. D. Kenna, Cornelis Blauwendraat, John C. Hartley, Jane F. Turton, Paul Aurora, and Garth L. J. Dixon. Molecular Fingerprinting of Mycobacterium abscessus Strains in a Cohort of Pediatric Cystic Fibrosis Patients. J. Clin. Microbiol., May 2012; 50: 1758 – 1761.

Customer Comments

New Method for Ectomycorrhizal Roots

The MO BIO UltraClean^® Microbial DNA Kit works well for us. We have been trying to isolate DNA from ectomycorrhizal roots. Published methods for this are available, but they are slow and may involve highly toxic chemicals. When isolating DNA from pine ectomycorrhizas, we use the Microbial DNA Kit in conjunction with IRS (Inhibitor Removal Solution, available from MO BIO) and obtain PCR-ready DNA. No grinding or freezing is necessary. The process is simple and rapid.

Roger Koide
The Pennsylvania State University

Kit Components