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UltraClean® -htp 96 Well Microbial DNA Kit

Overview

For high throughput isolation of DNA from 96 microbial cultures in 1.5 hours
Order
Description Catalog # Price
4 x 96 preps 10196-4 $1,048.00    
12 x 96 preps 10196-12 $2,923.00    

Features

- Process 96 microbial samples in 1.5 hours.

- Yields DNA greater than 50 kb in length ready for PCR, restriction digests and other downstream applications.

- Isolate DNA from yeast, fungi, Gram-negative and Gram-positive bacteria with a silica spin plate format.

- Centrifuge and centrifuge/vacuum protocols provided.

- All tubes and reagents required are included in kit and ready to use.

- No hazardous organic solvents or enzymes required.

Description

The UltraClean-htp® 96 Well Microbial DNA Isolation Kit is designed to isolate high quality genomic DNA from microorganisms in a 96 well format. This kit requires a plate centrifuge and a 96 well Plate Shaker. Both centrifuge and centrifuge/vacuum protocols are provided. A variety of microorganisms including bacterial spores and fungal types have been tested successfully with this kit.

The microbial cells are added to a bead beating plate containing beads, bead solution and lysis solution. The principal is to lyse the microorganisms by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action using a 96 well Plate Shaker. From the lysed cells, the released DNA is bound to a silica spin plate. The plate is washed, and the DNA is recovered in certified DNA-free Tris buffer.

Research and Development

Consistent DNA Yields with
UltraClean®-htp 96 Well Microbial DNA Isolation Kit
 
 
    1 2 3 4 5 6 7 8 9  

 

 

Lane 1: Marker
Lane 2: E. coli
Lane 3: E. faecalis
Lane 4: B. subtilis
Lane 5: P. fluorescens
Lane 6: C. albicans
Lane 7: C. albicans
Lane 8: S. cerevisiae
Lane 9: S. cerevisiae

 

DNA was purified from various microorganisms and displayed on 1.2% TAE agarose gel.

UltraClean® -htp 96 Well Microbial DNA Kit
Click over image to enlarge

MSDS

10196

Protocol

10196

Specifications

Format Silica Spin Plate
Method Bead Beating
Binding Capacity Up to 20 µg per well
Throughput 96 samples
Time 1.5 hours


Microorganisms tested
with UltraClean Kit
  • B. subtilis
  • B. pumulis
  • E. coli
  • E. faecalis
  • P. aeruginosa
  • M. luteus
  • S. aureus
  • L. casei
  • A. niger
  • C. albicans
  • S. cerevisiae

 

Storage Conditions

Store at room temperature for up to 2 years.

Publications

Kit Components

Component 10196-4 10196-12
Bead Plates 4 12
Microbead Solution 1 x 127 ml 1x 381 ml
Solution MD1 1 x 26 ml 1 x 78 ml
Solution MD2 1 x 43 ml 1 x 129 ml
Solution MD3 1 x 340 ml 1 x 1020 ml
Solution MD4 12 x 30 ml 36 x 30 ml
Solution MD5 1 x 43 ml 1 x 129 ml
Spin Plates 4 12
2 ml Collection Plates 12 36
1 ml Collection Plates 4 12
Microplates 4 12
Sealing Tape 4 12
Centrifuge Tape 24 72
Round Well Mats 8 24