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UltraClean® -htp 96 Well Microbial DNA Kit
Overview
For high throughput isolation of DNA from 96 microbial cultures in 1.5 hoursOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 4 x 96 preps | 10196-4 | Call for price | |
| 12 x 96 preps | 10196-12 | Call for price | |
Features
- Process 96 microbial samples in 1.5 hours.
- Yields DNA greater than 50 kb in length ready for PCR, restriction digests and other downstream applications.
- Isolate DNA from yeast, fungi, Gram-negative and Gram-positive bacteria with a silica spin plate format.
- Centrifuge and centrifuge/vacuum protocols provided.
- All tubes and reagents required are included in kit and ready to use.
- No hazardous organic solvents or enzymes required.
Description
The UltraClean-htp® 96 Well Microbial DNA Isolation Kit is designed to isolate high quality genomic DNA from microorganisms in a 96 well format. This kit requires a plate centrifuge and a 96 well Plate Shaker. Both centrifuge and centrifuge/vacuum protocols are provided. A variety of microorganisms including bacterial spores and fungal types have been tested successfully with this kit.
The microbial cells are added to a bead beating plate containing beads, bead solution and lysis solution. The principal is to lyse the microorganisms by a combination of heat, detergent, and mechanical force against specialized beads. The cellular components are lysed by mechanical action using a 96 well Plate Shaker. From the lysed cells, the released DNA is bound to a silica spin plate. The plate is washed, and the DNA is recovered in certified DNA-free Tris buffer.
Research and Development
UltraClean®-htp 96 Well Microbial DNA Isolation Kit
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| Lane 1: Marker Lane 2: E. coli Lane 3: E. faecalis Lane 4: B. subtilis Lane 5: P. fluorescens |
Lane 6: C. albicans Lane 7: C. albicans Lane 8: S. cerevisiae Lane 9: S. cerevisiae |
DNA was purified from various microorganisms and displayed on 1.2% TAE agarose gel.
Click over image to enlarge
MSDS
Protocol
Specifications
| Format | Silica Spin Plate |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per well |
| Throughput | 96 samples |
| Time | 1.5 hours |
with UltraClean Kit |
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Storage Conditions
Store at room temperature for up to 2 years.Publications
- Zhang, L., Srinivasan, U., Marrs, C.F., Ghosh, D., Gilsdorf, J.R., Foxman, B. Library On A Slide For Bacterial Comparative Genomics. BMC Microbiology. 4(12) (2004).
Kit Components
| Component | 10196-4 | 10196-12 |
|---|---|---|
| Bead Plates | 4 | 12 |
| Microbead Solution | 1 x 127 ml | 1x 381 ml |
| Solution MD1 | 1 x 26 ml | 1 x 78 ml |
| Solution MD2 | 1 x 43 ml | 1 x 129 ml |
| Solution MD3 | 1 x 340 ml | 1 x 1020 ml |
| Solution MD4 | 12 x 30 ml | 36 x 30 ml |
| Solution MD5 | 1 x 43 ml | 1 x 129 ml |
| Spin Plates | 4 | 12 |
| 2 ml Collection Plates | 12 | 36 |
| 1 ml Collection Plates | 4 | 12 |
| Microplates | 4 | 12 |
| Sealing Tape | 4 | 12 |
| Centrifuge Tape | 24 | 72 |
| Round Well Mats | 8 | 24 |
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