PowerMicrobial® Midi DNA Isolation Kit

Overview

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Features

- Zero post-extraction cell survival provides safety in the lab and is ideal for pathogens.

- Midi kit isolates up to 100 µg of DNA

- 100% cell lysis efficiency permits DNA isolation from challenging microorganisms; spores, Gram-positive and Gram-negative bacteria, yeast and fungi.

- Utilizes phenol: chloroform in combination with bead beating lysis for maximum performance.

- Purify DNA from microbial cultures in less than 2 hours.

- Streamlined protocol utilizes common laboratory equipment.

- Requires MO BIO Vortex Adapter for cell lysis.

Description

The PowerMicrobial^® Midi DNA Isolation Kit is designed to isolate DNA from microorganisms grown in up to 50 ml of culture with a density of at least 108 cells/ml. The versatility of the PowerMicrobial^® Midi DNA Isolation Kit efficiently isolates DNA from a wide range of microorganisms; Gram-positive, Gram-negative, yeast and fungi. Typical yields will depend on the type of microorganism and culture cell density. The maximum binding capacity of the spin columns is 100 µg DNA.

The success of the PowerMicrobial^® Midi DNA Isolation Kit is due to the highly effective lysing method. This unique bead-beating method together with an optimized phenol: chloroform* reagent results in both maximum yields and sterile DNA in all tested microorganisms. Purified DNA is free of viable microbial cells or spores and can be used safely in the laboratory – even if originated from pathogenic organisms. (Note: Always verify safety of DNA by post extraction plate counts). This kit utilizes common laboratory equipment and simply requires a vortex and vortex adapter for maximum effectiveness.

The microbial cells, resuspended in Bead Solution, are added to a bead beating tube containing beads. The principal is to lyse the microorganisms by a combination of detergent, phenol, and mechanical force using specialized beads. The cellular components are inactivated and lysed by a combination of phenol and mechanical action using a specially designed MO BIO Vortex Adapter on a standard vortex. From the lysed cells, the released DNA is bound to silica membranes in a spin filter format. The filter is washed, and the DNA is eluted in a sterile 10 mM Tris buffer. The resultant DNA is free of viable microbial cells, which is especially important when working with pathogenic microorganisms.

MSDS

 12225

Protocol

12225

Specifications

Storage Conditions

Kit Components