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PowerLyzer™ UltraClean® Microbial DNA Isolation Kit
Overview
For isolation of high quality DNA from microbial cultures and optimized for use with bead based homogenizers like the PowerLyzer™ 24.Order
| Description | Catalog # | Price | |
|---|---|---|---|
| 50 preps | 12255-50 | $111.00 |
|
Features
- Rapidly purifies DNA from microbial cultures in 20 minutes
- Optimized for use with bead based homogenizers such as the PowerLyzer™ 24
- Yields high quality DNA up to 50 kb plus that is ready for PCR, restriction digests and other downstream applications
- Isolates DNA from yeast, fungi, Gram-negative and Gram-positive bacteria
- No hazardous organic solvents or enzymes required
Description
The PowerLyzer™ UltraClean® Microbial DNA Isolation kit is designed to isolate high quality genomic DNA from 1.8 ml of microbial culture. A variety of microorganisms including bacterial spores and fungal types have been tested successfully with this kit.
The principal of this kit is to lyse the microorganisms by a combination of heat, detergent, and mechanical force against specialized beads. The cells are lysed using our specially designed PowerLyzer™ 24 bead based homogenizer. The released DNA is then bound to a silica spin filter, washed and the DNA is recovered in certified DNA-free Tris buffer. The PowerLyzer™ UltraClean® Microbial DNA Isolation Kit requires our PowerLyzer™ 24 homogenizer for maximum cell lysis efficiency.
NEW: Speed your protocol up to 20% in using our PowerVac™ Manifold Mini System!
Sample Kit
Click to try a sample.
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MSDS
Protocol
Specifications
View the full PowerLyzer™ UltraClean®
Microbial DNA Application Note
| Format | Silica Spin Filter Tubes |
|---|---|
| Method | Bead Beating |
| Binding Capacity | Up to 20 µg per filter |
| Through-put | 1 - 24 samples |
| Time | 20 minutes |
Storage Conditions
Store at room temperature for up to 2 years.
Kit Components
| Component | 12224-50 |
|---|---|
| Solution MD1 Solution MD2 Solution MD3 Solution MD4 Solution MD5 Glass Microbead Tubes Microbead Solution Spin Filters In Tubes Collection Tubes |
1 x 2.75 ml 1 x 5.5 ml 1 x 50 ml 1 x 16.5 ml 1 x 3 ml 50 1 x 16.5 ml 50 200 |
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