BiOstic® Bacteremia DNA Isolation Kit
Isolate bacterial DNA from cultured blood.
- Sensitive detection permits use with low bacterial cell counts
- Removes all inhibitors from blood and culture medium for robust amplification of bacterial DNA
- Easy-to-use method requires no special equipment
- Rigorous lysis protocol ensures complete lysis of tough organisms and high yield of microbial DNA
- Fast protocol has no prolonged enzyme digestions so DNA in isolated in less than 45 minutes from start to finish
The BiOstic® Bacteremia DNA Isolation Kit enables efficient purification of bacterial DNA from cultured blood, fecal and cervical swabs while completely removing PCR inhibitors common in blood, swabs and culture medium.
Bacterial genotyping from cultured blood can be difficult due to the presence of PCR inhibitors such as charcoal used for the removal of antibiotics from patient blood (e.g. BacT/ALERT™ SA tubes).
The BiOstic® Bacteremia DNA Isolation Kit utilizes our novel, patented Inhibitor Removal Technology® (IRT) combined with mechanical bead beating lysis to isolate pure genomic DNA that is free of inhibitory substances and ready to use in bacterial genotyping assays such as the Bacterial Barcodes Rep-PCR assays and other multiplex PCR or real-time PCR assays.
The BiOstic® Bacteremia DNA Isolation Kit has been successfully tested on Gram - and Gram + bacteria.
Click to try a sample.
Click over image to enlarge
||Silica Spin Filter Tubes
||1.8 ml of cultured blood
||Up to 20 µg per spin filter
||Vortex and centrifuge
Store at room temperature for up to 2 years.
- A. Lerner, J. Romano, I. Chmelnitsky, S. Navon-Venezia, R. Edgar, Y. Carmelia. Rectal Swabs Are Suitable for Quantifying the Carriage Load of KPCProducing Carbapenem-Resistant Enterobacteriaceae. Antimicrobial Agents and Chemotherapy p. 1474–1479 March 2013 Volume 57 Number 3
- Jeanne M. Marrazzo, Tina L. Fiedler, Sujatha Srinivasan, Katherine K. Thomas, Congzhou Liu, Daisy Ko, Hu Xie, Misty Saracino, and David N. Fredricks. Extravaginal Reservoirs of Vaginal Bacteria as Risk Factors for Incident Bacterial Vaginosis. The Journal of Infectious Disease, May 2012; 205: 1580 - 1588.
- Edward D. Ziga, Todd Druley, and Carey-Ann D. Burnham Herbaspirillum Species Bacteremia in a Pediatric Oncology Patient. J. Clin. Microbiol., Nov 2010; 48: 4320 - 4321.