BiOstic® Bacteremia DNA Isolation Kit

Overview

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Features

• Sensitive detection permits use with low bacterial cell counts

• Removes all inhibitors from blood and culture medium for robust amplification of bacterial DNA

• Easy-to-use method requires no special equipment

• Rigorous lysis protocol ensures complete lysis of tough organisms and high yield of microbial DNA

• Fast protocol has no prolonged enzyme digestions. Isolate DNA in less than 45 minutes from start to finish

Description

The BiOstic^® Bacteremia DNA Isolation Kit enables efficient purification of bacterial DNA from cultured blood, fecal and cervical swabs while completely removing PCR inhibitors common in blood, swabs and culture medium.

Bacterial genotyping from cultured blood can be difficult due to the presence of PCR inhibitors such as charcoal used for the removal of antibiotics from patient blood (e.g. BacT/ALERT™ SA tubes).

The BiOstic^® Bacteremia DNA Isolation Kit utilizes our novel, patented Inhibitor Removal Technology^® (IRT) combined with mechanical bead beating lysis to isolate pure genomic DNA that is free of inhibitory substances and ready to use in bacterial genotyping assays such as the Bacterial Barcodes Rep-PCR assays and other multiplex PCR or real-time PCR assays.

The BiOstic^® Bacteremia DNA Isolation Kit has been successfully tested on Gram - and Gram + bacteria.

Sample Kit

Click to try a sample.

MSDS

12240

Protocol

12240

Specifications

Storage Conditions

Publications

• Jeanne M. Marrazzo, Tina L. Fiedler, Sujatha Srinivasan, Katherine K. Thomas, Congzhou Liu, Daisy Ko, Hu Xie, Misty Saracino, and David N. Fredricks. Extravaginal Reservoirs of Vaginal Bacteria as Risk Factors for Incident Bacterial Vaginosis. The Journal of Infectious Disease, May 2012; 205: 1580 - 1588.
• Edward D. Ziga, Todd Druley, and Carey-Ann D. Burnham Herbaspirillum Species Bacteremia in a Pediatric Oncology Patient. J. Clin. Microbiol., Nov 2010; 48: 4320 - 4321.
• Marrazzo, J.M., Fiedler, T.L., Srinivasan, S., Thomas, K.K., Liu, C., Ko, D., Xie, H., Saracino, M., and Fredricks, D.N. Extravaginal Reservoirs of Vaginal Bacteria as Risk Factors for Incident Bacterial Vaginosis. J Infect Dis. 205(10): 1580-1588 (2012).

 

Kit Components