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BiOstic® Bacteremia DNA Isolation Kit

Overview

Isolate bacterial DNA from cultured blood.
Order
Description Catalog # Price
50 preps 12240-50 $162.00    

Features

  • Sensitive detection permits use with low bacterial cell counts
  • Removes all inhibitors from blood and culture medium for robust amplification of bacterial DNA
  • Easy-to-use method requires no special equipment
  • Rigorous lysis protocol ensures complete lysis of tough organisms and high yield of microbial DNA
  • Fast protocol has no prolonged enzyme digestions so  DNA in isolated in less than 45 minutes from start to finish

Description

The BiOstic® Bacteremia DNA Isolation Kit enables efficient purification of bacterial DNA from cultured blood, fecal and cervical swabs while completely removing PCR inhibitors common in blood, swabs and culture medium.


Bacterial genotyping from cultured blood can be difficult due to the presence of PCR inhibitors such as charcoal used for the removal of antibiotics from patient blood (e.g. BacT/ALERT™ SA tubes).

The BiOstic® Bacteremia DNA Isolation Kit utilizes our novel, patented Inhibitor Removal Technology® (IRT) combined with mechanical bead beating lysis to isolate pure genomic DNA that is free of inhibitory substances and ready to use in bacterial genotyping assays such as the Bacterial Barcodes Rep-PCR assays and other multiplex PCR or real-time PCR assays.

The BiOstic® Bacteremia DNA Isolation Kit has been successfully tested on Gram - and Gram + bacteria.

Sample Kit

Click to try a sample.

BiOstic®  Bacteremia DNA Isolation Kit
Click over image to enlarge

MSDS

12240

Protocol

12240

Specifications

Format Silica Spin Filter Tubes
Method Bead Beating
Sample Size 1.8 ml of cultured blood
Binding Capacity Up to 20 µg per spin filter
Purity
 
A260/280: 1.7-1.9
A260/230: >2.0
Throughput 1-24 samples
Time 45 minutes
Equipment Required Vortex and centrifuge

Storage Conditions

Store at room temperature for up to 2 years.

Publications

Kit Components

Component 12240-50
Solution CB1
Solution CB2
Solution CB3
Solution CB4
Solution CB5
2 ml MicroBead Tubes
Spin Filters
2 ml Collection Tubes
25 ml
6 ml
2 x 28 ml
2 x 30 ml
6 ml
50 Bead Tubes
50 Spin Filter units
250 x 2 ml tubes