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UltraClean® Midi Plasmid Prep Kit
Overview
For isolation of plasmid DNA from 25 to 50 ml of bacterial cultureOrder
| Description | Catalog # | Price | |
|---|---|---|---|
| 20 preps | 12700-20 | $132.00 |
|
| 50 preps | 12700-50 | $280.00 |
|
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UltraClean® 6 Minute Mini Plasmid Prep Kit
Features
- Rapid protocol isolates high quality plasmid DNA in 30 minutes
- Yields high quality DNA, ready for restriction digest, sequencing, labeling or PCR
- Easy format utilizes large silica spin filters
- High yields from standard or high nutrient broth culture
Description
The UltraClean® Midi Plasmid Prep Kit is designed to isolate plasmid DNA from large culture volumes of E. coli. The Midi Kit uses the same silica spin filter technology employed in the UltraClean® 6 Minute Mini Plasmid Prep Kit. Unlike other kits, this kit can be used with LB Broth culture and high nutrient broth culture at the following media volumes: up to 50 ml of an LB broth culture and up to 25 ml of a high nutrient broth culture. The procedure is to lyse cells with an improved set of alkaline lysis reagents. The DNA is bound to a silica spin filter, washed once and released into a low volume of elution buffer. The DNA is concentrated enough to be used directly in automated sequencing. The DNA is also ready for restriction digests, labeling, PCR and other downstream applications.
Sample Kit
Click to try a sample.
Research and Development
CORE Sequencing Center using DNA purified with the
UltraClean® Midi Plasmid Prep Kit
Click over image to enlarge
Protocol
Specifications
| Format | 50 ml Silica Spin Filter Tubes |
|---|---|
| Method | Alkaline Lysis |
| Binding Capacity | Up to 1 mg per filter |
| Media Volume | TB DRY®, 250 ml LB Broth, 500 ml |
| Throughput | 1 - 4 samples |
| Time | 45 minutes |
Storage Conditions
Store at room temperature for up to 2 years.Publications
- Wakelin, S.A., Colloff, M.J., Kookana, R.S. Effect of Waterwaste Treatment Plant Effluent on Microbial Function and Community Structure in the Sediment of a Freshwater Stream with Variable Seasonal Flow . Applied Environmental Microbiology. (74)9: 2659-2668 (2008).
Kit Components
| Component | 12700-20 | 12700-50 |
|---|---|---|
| Solution 1 Solution 2 Solution 3 Solution 4 Solution 5 Spin Filters 15 ml Tubes 50 ml Tubes |
1 x 10 ml 1 x 18 ml 1 x 52 ml 2 x 30 ml 1 x 22 ml 20 60 20 |
1 x 22.5 ml 1 x 44 ml 1 x 130 ml 1 x 80 ml 1 x 55 ml 50 150 50 |
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High nutrient media ideal for low copy plasmids.
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For isolation of plasmid DNA from 250 to 500 ml of bacterial culture
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For high throughput plasmid minipreps in less than 45 minutes
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For isolation of high quality DNA using a familiar miniprep protocol
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Fastest kit available for high quality minipreps in just 6 minutes
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